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Product Info Summary
| SKU: | M03651 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | FACS |
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Product info
Product Name
Anti-hCD50 Antibody (Monoclonal, 101-1D2)
SKU/Catalog Number
M03651
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-hCD50 Antibody (Monoclonal, 101-1D2) (Catalog# M03651). Tested in FACS application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-hCD50 Antibody (Monoclonal, 101-1D2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03651)
Host
Mouse
Contents
100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
101-1D2
Isotype
IgG2a Kappa
Immunogen
Stimulated human leukocytes
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M03651 is reactive to ICAM3 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
59.5 kDa
Background of ICAM3
Recognizes a protein of 42-46kDa, identified as MAGE-1. This monoclonal antibody does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03651 is guaranteed for FACS Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
FACS: 1-2 µg/10^6 cells
Validation Images & Assay Conditions
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Cell Surface flow analysis of hCD50 in PBMC (Lymphocyte gated) using 1 &mu
Specific Publications For Anti-hCD50 Antibody (Monoclonal, 101-1D2) (M03651)
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