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Product Info Summary
| SKU: | A01025-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CD209/Dc Sign Antibody
SKU/Catalog Number
A01025-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD209/Dc Sign Antibody catalog # A01025-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD209/Dc Sign Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01025-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from CD209 antigen at AA range: 261-310
Reactive Species
A01025-1 is reactive to CD209 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
45.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01025-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of mouse brain cells using CD209 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-CD209/Dc Sign Antibody (A01025-1)
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Customer Q&As
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15 Customer Q&As for Anti-CD209/Dc Sign Antibody
Question
Does A01025-1 anti-CD209/Dc Sign antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
As indicated on the product datasheet, A01025-1 anti-CD209/Dc Sign antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-10
Question
I see that the anti-CD209/Dc Sign antibody A01025-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-09
Question
My colleagues were satisfied with the WB result of your anti-CD209/Dc Sign antibody. However we have been able to see positive staining in adrenal gland isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is CD209 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
From what I have seen in literature, adrenal gland does express CD209. Generally CD209 expresses in isoform 1: cell membrane. Regarding which tissues have CD209 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 1518869
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-18
Question
We are currently using anti-CD209/Dc Sign antibody A01025-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
The anti-CD209/Dc Sign antibody (A01025-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-27
Question
I am interested in to test anti-CD209/Dc Sign antibody A01025-1 on human uterus for research purposes, then I may be interested in using anti-CD209/Dc Sign antibody A01025-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
The products we sell, including anti-CD209/Dc Sign antibody A01025-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-21
Question
We have been able to see staining in mouse adrenal gland. Are there any suggestions? Is anti-CD209/Dc Sign antibody supposed to stain adrenal gland positively?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
From literature adrenal gland does express CD209. From Uniprot.org, CD209 is expressed in adrenal gland, placenta, uterus, among other tissues. Regarding which tissues have CD209 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 1518869
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-03-12
Question
I was wanting to use your anti-CD209/Dc Sign antibody for WB for human uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human uterus identification?
Verified Customer
Verified customer
Asked: 2018-07-10
Answer
As indicated on the product datasheet, A01025-1 anti-CD209/Dc Sign antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-10
Question
Would anti-CD209/Dc Sign antibody A01025-1 work for WB with uterus?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
According to the expression profile of uterus, CD209 is highly expressed in uterus. So, it is likely that anti-CD209/Dc Sign antibody A01025-1 will work for WB with uterus.
Boster Scientific Support
Answered: 2018-05-08
Question
Is a blocking peptide available for product anti-CD209/Dc Sign antibody (A01025-1)?
Verified Customer
Verified customer
Asked: 2017-07-14
Answer
We do provide the blocking peptide for product anti-CD209/Dc Sign antibody (A01025-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-07-14
Question
Can you help my question with product A01025-1, anti-CD209/Dc Sign antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01025-1 anti-CD209/Dc Sign antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-02
Question
Please see the WB image, lot number and protocol we used for uterus using anti-CD209/Dc Sign antibody A01025-1. Please let me know if you require anything else.
B. Williams
Verified customer
Asked: 2016-08-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-08-15
Question
Is there a BSA free version of anti-CD209/Dc Sign antibody A01025-1 available?
B. Parker
Verified customer
Asked: 2016-06-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD209/Dc Sign antibody A01025-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-06-22
Question
My question regards using your anti-CD209/Dc Sign antibody for intracellular signal transduction studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
A. Jones
Verified customer
Asked: 2015-08-21
Answer
We appreciate your inquiry. This A01025-1 anti-CD209/Dc Sign antibody is tested on mouse brain, brain cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-08-21
Question
Is this A01025-1 anti-CD209/Dc Sign antibody reactive to the isotypes of CD209?
K. Carter
Verified customer
Asked: 2014-05-22
Answer
The immunogen of A01025-1 anti-CD209/Dc Sign antibody is Synthesized peptide derived from the Internal region of human CD209. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-05-22
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for uterus using anti-CD209/Dc Sign antibody A01025-1. Let me know if you need anything else.
M. Yang
Verified customer
Asked: 2013-03-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-03-22
