Anti-HDAC-4 (internal) Antibody

Product Name

Anti-HDAC-4 (internal) Antibody

View all HDAC4 Antibodies

SKU/Catalog Number

A00971-2

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-HDAC-4 (internal) Antibody (Catalog # A00971-2). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Primate.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening. (Ship on dry ice.)

Cite This Product

Anti-HDAC-4 (internal) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00971-2)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol

Clonality

Polyclonal

Isotype

IgG

Immunogen

HDAC4 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 600-650aa of human HDAC4.

Reactive Species

A00971-2 is reactive to HDAC4 in Human, Mouse, Rat, Primate

Observed Molecular Weight

42 kDa

Calculated molecular weight

119.0 kDa

Background of HDAC4

HDAC4 is a 140 kD class II histone deacetylase similar to the yeast HDA1 protein. This nuclear protein operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC4 uses active nuclear export to shuttle between the nucleus and the cytoplasm. HDAC4 is modified by phosphorylation by CaM kinase I or IV. HDAC4 has been reported to interact with 14-3-3 in the cytoplasm and MEF2A, HDAC3, NcoR and SMRT in the nucleus. Anti-HDAC4 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00971-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:60,000
IHC: 1 mg/mL
IF Microscopy: 1:100-1:500
WB: 1 mg/mL
Anti-HDAC4 antibody has been tested in Western Blot and is useful for ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~140 kDa corresponding to the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information HDAC4

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