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Product Info Summary
| SKU: | M02177 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HDAC9 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02177
BM4726 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HDAC9 Rabbit Monoclonal Antibody catalog # M02177. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDAC9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02177)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HEF-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HDAC9
Reactive Species
M02177 is reactive to HDAC9 in Human, Rat
Observed Molecular Weight
160 kDa
Calculated molecular weight
111.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02177 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: K562 cell
IHC: human brain tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of HDAC9 expression in K562 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human brain, using HDAC9 Antibody.
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Immunofluorescent analysis of Hela cells, using HDAC9 Antibody.
Specific Publications For Anti-HDAC9 Rabbit Monoclonal Antibody (M02177)
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1 Customer Q&As for Anti-HDAC9 Rabbit Monoclonal Antibody
Question
We are currently using anti-HDAC9 Rabbit Monoclonal antibody M02177 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on canine tissues as well?
R. Williams
Verified customer
Asked: 2015-12-18
Answer
The anti-HDAC9 Rabbit Monoclonal antibody (M02177) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-18
