Click image to see more details
Product Info Summary
| SKU: | A05895-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IP, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-HIP2/LIG/UBE2K Antibody Picoband®
SKU/Catalog Number
A05895-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HIP2/LIG/UBE2K Antibody Picoband® catalog # A05895-2. Tested in ELISA, Flow Cytometry, IP, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HIP2/LIG/UBE2K Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05895-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HIP2/LIG/UBE2K recombinant protein (Position: D33-E195).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05895-2 is reactive to UBE2K in Human, Monkey, Mouse, Rat
Observed Molecular Weight
22 kDa
Calculated molecular weight
22.4 kDa
Background of UBE2K
The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05895-2 is guaranteed for ELISA, Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat, Monkey
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: monkey COS-7 whole cell, human HEK293 whole cell, rat brain tissue, rat testis tissue, mouse brain tissue, mouse testis tissue
IP: HepG2 cell
FCM: RH35 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HIP2/LIG/UBE2K using anti-HIP2/LIG/UBE2K antibody (A05895-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: monkey COS-7 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIP2/LIG/UBE2K antigen affinity purified polyclonal antibody (Catalog # A05895-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIP2/LIG/UBE2K at approximately 22 kDa. The expected band size for HIP2/LIG/UBE2K is at 22 kDa.
Click image to see more details
Immunoprecipitating (IP) HIP2/LIG/UBE2K in HepG2 whole cell lysate.
Western blot analysis of HIP2/LIG/UBE2K using anti-HIP2/LIG/UBE2K antibody (A05895-2);
Lane 1: HepG2 whole cell lysates (30ug);
Lane 2: Rabbit control IgG instead of anti-HIP2/LIG/UBE2K antibody in HepG2 whole cell lysate;
Lane 3: anti-HIP2/LIG/UBE2K antibody (2μg) + HepG2 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HIP2/LIG/UBE2K antigen affinity purified polyclonal antibody (A05895-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for HIP2/LIG/UBE2K at approximately 22 kDa. The expected band size for HIP2/LIG/UBE2K is at 22 kDa.
Click image to see more details
Flow Cytometry analysis of RH35 cells using anti-HIP2/LIG/UBE2K antibody (A05895-2).
Overlay histogram showing RH35 cells stained with A05895-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIP2/LIG/UBE2K Antibody (A05895-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-HIP2/LIG/UBE2K Antibody Picoband® (A05895-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-HIP2/LIG/UBE2K Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-HIP2/LIG/UBE2K Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
