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GNG4 antibody (M30975-1) performs well in WB and shows reactivity with All species.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 19 Publications

m30975 1 his tag primary antibodies wb testing 1

Western blot analysis of His Tag using anti-His Tag antibody (M30975-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse His Tag protein 10ng,
Lane 1: recombinant mouse His Tag protein 5ng,
Lane 1: recombinant mouse His Tag protein 2.5ng,
Lane 1: recombinant mouse His Tag protein 1.25ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-His Tag antigen affinity purified monoclonal antibody (Catalog # M30975-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.

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Anti-His Tag Antibody Picoband® (monoclonal, 19b10)

GNG4 antibody

19 Publications

Reactive Species	Host	Application
All species	Mouse	WB

Product Information

Product Name

Anti-His Tag Antibody Picoband® (monoclonal, 19b10)
View all GNG4 Antibodies

Form

Lyophilized

Uniprot

P50150

Gene ID

2786

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

19b10

Isotype

Mouse IgG2b

Immunogen

A synthetic peptide corresponding to a sequence of His Tag.

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Observed Molecular Weight

12 kDa

Calculated Molecular Weight

8.4 kDa

Background of GNG4

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4 is a protein that in humans is encoded by the GNG4 gene. This gene encodes the gamma subunit of the heterotrimeric G-proteins that are comprised of alpha, beta and gamma subunits. Upon activation by G protein-coupled receptors, the beta-gamma heterodimer dissociates from the alpha subunit to activate downstream signaling events. Alternate splicing results in multiple transcript variants.

Cite This Product

Anti-His Tag Antibody Picoband® (monoclonal, 19b10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30975-1)

Publications

Surface-displayed multivalent PRRSV tandem antigen on Bacillus subtilis spores induces mucosal immune responses in mice

Published: 2026-05-09

Journal: JOURNAL OF APPLIED MICROBIOLOGY

Application: WB

Impact Factor: 2.80

Species: Bacillus subtilis

PMID: 42104959

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Anti-His Tag Antibody Picoband® (monoclonal, 19b10)

GNG4 antibody

Overview

Recommend Dilution

Product Information

Publications

Surface-displayed multivalent PRRSV tandem antigen on Bacillus subtilis spores induces mucosal immune responses in mice

Identification and evaluation of cross-protection efficiency of the conserved antigens of Salmonella Enteritidis

Rewiring of cell morphology by Small protein B enhances stress tolerance and colonization in Aeromonas veronii under adverse conditions

Dual effects of tomato chlorosis virus on its whitefly vector and its host plant to facilitate viral spread

Surface Display of Duck Hepatitis A Virus Type 1 VP1 Protein on Bacillus subtilis Spores Elicits Specific Systemic and Mucosal Immune Responses on Mice

Mycoplasma synoviae LP78 is a fibronectin/plasminogen binding protein, putative adhesion, and potential diagnostic antigen

Oral Immunization with Attenuated Salmonella Choleraesuis Expressing the FedF Antigens Protects Mice against the Shiga-Toxin-Producing Escherichia coli Challenge

Development and evaluation of a multi-epitope subunit vaccine against Mycoplasma synoviae infection

Immune efficacy of carbon nanotubes recombinant subunit vaccine against largemouth bass ulcerative syndrome virus

Carbon nanotubes-loaded subunit vaccine can increase protective immunity against rhabdovirus infections of largemouth bass (Micropterus Salmoides)

Identification and functional characterization of grass carp (Ctenopharyngodon idella) tumor necrosis factor receptor 2 and its soluble form with potentiality for targeting inflammation

Molecular and biological characterization of gamma-interferon-inducible lysosomal thiol reductase in silver carp (Hypophthalmichthys molitrix)

Surface displaying of swine IgG1 Fc enhances baculovirus-vectored vaccine efficacy by facilitating viral complement escape and mammalian cell transduction

The non-conserved region of MRP is involved in the virulence of Streptococcus suis serotype 2

Characterization of a novel Cry8Ea3-binding V-ATPase Subunit A in Holotrichia parallela

Glucosidase II β-subunit, a novel substrate for caspase-3-like activity in rice, plays as a molecular switch between autophagy and programmed cell death

Identification of a New Peritrophic Membrane Protein from Larval Holotrichia parallela (Coleoptera: Motschulsky)

Soluble Expression of Recombinant Human Smp30 for Detecting Serum Smp30 Antibody Levels in Hepatocellular Carcinoma Patients

Molecular structure of the largemouth bass (Micropterus salmoides) Myf5 gene and its effect on skeletal muscle growth

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