Anti-His Tag Antibody Picoband™ (monoclonal, 19b10)

Product Name

Anti-His Tag Antibody Picoband™ (monoclonal, 19b10)

View all G gamma4 Antibodies

SKU/Catalog Number

M30975-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-His Tag Antibody Picoband™ (monoclonal, 19b10) catalog # M30975-1. Tested in WB application. This antibody reacts with all species.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-His Tag Antibody Picoband™ (monoclonal, 19b10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30975-1)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

19b10

Isotype

Mouse IgG2b

Immunogen

A synthetic peptide corresponding to a sequence of His Tag.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M30975-1 is reactive to GNG4 in All species

Applications

M30975-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

12 kDa

Calculated molecular weight

8.389kDa

Background of G gamma4

Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4 is a protein that in humans is encoded by the GNG4 gene. This gene encodes the gamma subunit of the heterotrimeric G-proteins that are comprised of alpha, beta and gamma subunits. Upon activation by G protein-coupled receptors, the beta-gamma heterodimer dissociates from the alpha subunit to activate downstream signaling events. Alternate splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of His Tag using anti-His Tag antibody (M30975-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse His Tag protein 10ng,
Lane 1: recombinant mouse His Tag protein 5ng,
Lane 1: recombinant mouse His Tag protein 2.5ng,
Lane 1: recombinant mouse His Tag protein 1.25ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-His Tag antigen affinity purified monoclonal antibody (Catalog # M30975-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.

Gene/Protein Information For GNG4 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp547K1018; FLJ23803; FLJ34187; GNGT4; guanine nucleotide binding protein (G protein), gamma 4; guanine nucleotide binding protein 4; guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4 GNG4 G protein subunit gamma 4 guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4|guanine nucleotide binding protein (G protein), gamma 4|guanine nucleotide binding protein 4

For more info on GNG4, check out the GNG4 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GNG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the GNG4 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis