Product Info Summary
SKU: | A06243 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ChIP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H1.2 HIST1H1C Antibody
SKU/Catalog Number
A06243
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Histone H1.2 HIST1H1C Antibody catalog # A06243. Tested in WB,ICC,IF,IHC,ChIP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H1.2 HIST1H1C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06243)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human CA VIII.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06243 is reactive to H1-2 in Human, Mouse, Rat
Applications
A06243 is guaranteed for ChIP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
21.365kDa
Background of H1-2
Could be a T-cell-specific adapter protein involved in the control of T-cell activation. May play a role in the CD4-p56-LCK-dependent signal transduction pathway. Could also play an important role in normal and pathological angiogenesis. Could be an adapter protein that facilitates and regulates interaction of KDR with effector proteins important to endothelial cell survival and proliferation.
Spurkland A., J. Biol. Chem. 273:4539-4546(1998).
Wu L.-W., J. Biol. Chem. 275:6059-6062(2000).
Lee J.-S., Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:50-1:100
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Histone H1.2 HIST1H1C Antibody A06243
Western blot analysis of Histone H1.2 on different cell lysates using anti-Histone H1.2 antibody at 1/500 dilution.Positive control:
Lane 1: Hela
Lane 2: 293
Lane 3: MCF-7
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 2. Western blotting validation for Anti-Histone H1.2 HIST1H1C Antibody A06243
Western blot analysis of Histone H1.2 on Rat liver and Mouse lung tissue lysates using anti-Histone H1.2 antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For H1-2 (Source: Uniprot.org, NCBI)
Gene Name
H1-2
Full Name
Histone H1.2
Weight
21.365kDa
Superfamily
histone H1/H5 family
Alternative Names
Histone H1.2 H1-2 H1.2, H1C, H1F2, H1s-1, HIST1H1C H1.2 linker histone, cluster member histone H1.2|H1 histone family, member 2|histone 1, H1c|histone H1c|histone H1d|histone H1s-1|histone cluster 1 H1 family member c|histone cluster 1, H1c
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H1-2, check out the H1-2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H1-2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H1.2 HIST1H1C Antibody (A06243)
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No publications found for A06243
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