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Product Info Summary
| SKU: | M00241-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Vertebrates |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB, Luminex |
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Product info
Product Name
Anti-Histone H2AX H2AFX Rabbit Monoclonal Antibody, Clone#RM214
SKU/Catalog Number
M00241-2
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Histone H2AX H2AFX Rabbit Monoclonal Antibody, Clone#RM214 (Catalog # M00241-2). Tested in WB, ICC. ELISA, Luminex applications. This antibody reacts with Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H2AX H2AFX Rabbit Monoclonal Antibody, Clone#RM214 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00241-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM214
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human Histone H2AX
Cross-reactivity
This antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross-reactivity with other histone proteins. .
Reactive Species
M00241-2 is reactive to H2AFX in Vertebrates
Calculated molecular weight
15.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00241-2 is guaranteed for ELISA, ICC, WB, Luminex Boster Guarantee
Recommend Dilution
WB: 0.5 ug/mL – 2 ug/mL
ICC: 1 ug/mL - 2 ug/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.2 ug/mL – 1 ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of HeLa, HEK293, A375, and SK-MEL-2 whole cell lysates, using RM214 at 0.5 ug/mL, showed endogenous Histone H2AX in HeLa, HEK293, A375, and SK-MEL-2 cells.
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ICC result
Immunocytochemistry of HeLa cells, using Histone H2AX Rabbit mAb RM214 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Specific Publications For Anti-Histone H2AX H2AFX Rabbit Monoclonal Antibody, Clone#RM214 (M00241-2)
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