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HIST1H3A/B/C/D/E/F/G/H/I/J antibody (A12477-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 22 Publications

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a12477 2 histone h3 primary antibodies fcm testing 24

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Flow Cytometry analysis of RAW264.7 cells using anti-Histone H3 antibody (A12477-2).
Overlay histogram showing RAW264.7 cells stained with A12477-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Histone H3 Antibody (A12477-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband®

HIST1H3A/B/C/D/E/F/G/H/I/J antibody

22 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

HIST1H3A/B/C/D/E/F/G/H/I/J antibody was positively detected by WB in:

human HeLa cells, human HepG2 cells, human THP-1 cells, human U2OS cells, rat liver tissue, rat PC-12 cells, mouse liver tissue, mouse NIH3T3 cells

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

HIST1H3A/B/C/D/E/F/G/H/I/J antibody was positively detected by IHC in:

human colon adenocarcinoma, human esophageal squamous carcinoma tissue, human lung cancer tissue, human placenta tissue, human spleen tissue, human testicular seminoma tissue, mouse brain tissue, rat brain tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

HIST1H3A/B/C/D/E/F/G/H/I/J antibody was positively detected by ICC/IF in:

U2OS cell

HIST1H3A/B/C/D/E/F/G/H/I/J antibody was positively detected by FC in:

RAW264.7 cell, C6 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Product Information

Product Name

Anti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband®
View all HIST1H3A/B/C/D/E/F/G/H/I/J Antibodies

Form

Lyophilized

Uniprot

P68431

Gene ID

8350

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Histone H3 recombinant protein (Position: Q56—R117).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

17 kDa

Calculated Molecular Weight

15.4 kDa

Background of HIST1H3A/B/C/D/E/F/G/H/I/J

Histone H3.1 is a protein that in humans is encoded by the HIST1H3A gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Cite This Product

Anti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12477-2)

Protocols

Western Blot protocol for HIST1H3A/B/C/D/E/F/G/H/I/J antibody

Review Protocol

Immunohistochemistry (IHC) protocol for HIST1H3A/B/C/D/E/F/G/H/I/J antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HIST1H3A/B/C/D/E/F/G/H/I/J antibody

Review Protocol

Flow Cytometry (FC) protocol for HIST1H3A/B/C/D/E/F/G/H/I/J antibody

Review Protocol

Publications

Interfering with PTBP1 expression promotes anti-inflammatory macrophage polarization alongside altered activity of the miR-124/p38MAPK pathway

Published: 2026-07-21

Journal: TISSUE & CELL

Application: WB

Impact Factor: 3.10

Species: Mouse

PMID: 42497783

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Free Secondary Antibody

Anti-Histone H3 HIST1H3A/B/C/D/E/F/G/H/I/J Antibody Picoband®

HIST1H3A/B/C/D/E/F/G/H/I/J antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Interfering with PTBP1 expression promotes anti-inflammatory macrophage polarization alongside altered activity of the miR-124/p38MAPK pathway

Di-(2-ethylhexyl) Phthalate (DEHP) and Its Metabolite Promote Multiple Myeloma Progression and Bortezomib Resistance

Energy Stress-induced CircEPB41(2) Promotes Lipogenesis in Hepatocellular Carcinoma

Nanographene Oxide Promotes Angiogenesis by Regulating Osteoclast Differentiation and Platelet-Derived Growth Factor Secretion

Exploring the Anti-Inflammatory Effect of Tryptanthrin by Regulating TLR4/MyD88/ROS/NF-κB, JAK/STAT3, and Keap1/Nrf2 Signaling Pathways

Oxidized DJ-1 activates the p-IKK/NF-κB/Beclin1 pathway by binding PTEN to induce autophagy and exacerbate myocardial ischemia-reperfusion injury

JMJD3 regulate H3K27me3 modification via interacting directly with TET1 to affect spermatogonia self-renewal and proliferation

Role of TFEB-autophagy lysosomal pathway in palmitic acid induced renal tubular epithelial cell injury

Echinococcus granulosus cyst fluid inhibits inflammatory responses through inducing histone demethylase KDM5B in macrophages

Aucubin inhibits hepatic stellate cell activation through stimulating Nrf2/Smad7 axis

CD168+ macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A/β-catenin/YAP1 axis

CRP‑1 promotes the malignant behavior of hepatocellular carcinoma cells via activating epithelial‑mesenchymal transition and Wnt/β‑catenin signaling

Human Neural Stem Cell Secretome Inhibits Neuron Heme Uptake and Ferroptosis in Intracerebral Hemorrhage Through Nrf-2 Signaling Pathway

Naringenin protects against acute pancreatitis-associated intestinal injury by inhibiting NLRP3 inflammasome activation via AhR signaling

Alpha-lipoic acid impedes myocardial ischemia-reperfusion injury, myocardial apoptosis, and oxidative stress by regulating HMGB1 expression

lncRNA HOTTIP Recruits EZH2 to Inhibit PTEN Expression and Participates in IM Resistance in Chronic Myeloid Leukemia

Human Neural Stem Cell Secretome Inhibits Lipopolysaccharide-Induced Neuroinflammation Through Modulating Microglia Polarization by Activating Peroxisome Proliferator-Activated Receptor Gamma

Norisoboldine induces the development of Treg cells by promoting fatty acid oxidation-mediated H3K27 acetylation of Foxp3

HOXA10 inhibit the osteogenic differentiation of periodontal ligament stem cells by regulating β-catenin localization and DKK1 expression

KPNA2 promotes metabolic reprogramming in glioblastomas by regulation of c-myc

NiCoMnO4: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition

Potential neuroprotection of protodioscin against cerebral ischemia-reperfusion injury in rats through intervening inflammation and apoptosis

Review

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