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- Table of Contents
HIST1H4A antibody (M14495-2) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescent analysis of Hela cells, using Histone H4 Antibody.
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Anti-Histone H4 HIST1H4A Rabbit Monoclonal Antibody
HIST1H4A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
HIST1H4A antibody was positively detected by WB in:
human Hela whole cell, human PC-3 whole cell, human U20S whole cell, human Hacat whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse 3T3-L1 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HIST1H4A antibody was positively detected by IHC in:
human breast cancer tissue, human colorectal adenocarcinoma tissue, human liver cancer tissue, human lung squamous cell carcinoma tissue, human testicular germ cell tumor tissue, human placenta tissue, human thyroid cancer tissue, mouse brain tissue, rat brain tissue, human B-cell lymphoma, human colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HIST1H4A antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:2000-10000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Product Information
Product Name
Anti-Histone H4 HIST1H4A Rabbit Monoclonal Antibody
View all HIST1H4A Antibodies
View all HIST1H4A Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HDC-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H4
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
11 kDa
Calculated Molecular Weight
11.4 kDa
Cite This Product
Anti-Histone H4 HIST1H4A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14495-2)
Protocols
Western Blot protocol for HIST1H4A antibody
Immunohistochemistry (IHC) protocol for HIST1H4A antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HIST1H4A antibody
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Product Promise
We promise Anti-Histone H4 HIST1H4A Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M14495-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026