Anti-HLA-E Purified Monoclonal Antibody

Product Name

Anti-HLA-E Purified Monoclonal Antibody

View all HLA-E Antibodies

SKU/Catalog Number

M01944

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-HLA-E Purified Monoclonal Antibody (Catalog# M01944). Tested in WB, IHC-P application(s). This antibody reacts with Human, Primate.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-HLA-E Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01944)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MEM-E/02

Isotype

Mouse IgG1

Immunogen

Recombinant human HLA-E denaturated heavy chain. The antibody MEM-E/02 specifically reacts with an extracellular epitope on denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M01944 is reactive to HLA-E in Human, Primate

Observed Molecular Weight

42 kDa

Calculated molecular weight

40.1 kDa

Background of HLA-E

HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01944 is guaranteed for IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): 5-10 μg/ml; positive control: human placenta; heat antigen retrieval (sodium citrate), incubation with mAb: 1 hour / RT, detection DAB.

Validation Images & Assay Conditions

See full target information HLA-E

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