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Product Info Summary
| SKU: | A04392 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-RFX1 Antibody
SKU/Catalog Number
A04392
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RFX1 Antibody (Catalog # A04392). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
RFX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-RFX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04392)
Host
Rabbit
Contents
RFX1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1. The immunogen is located within the last 50 amino acids of RFX1.
Cross-reactivity
RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist; this antibody will detect both isoforms.
Reactive Species
A04392 is reactive to RFX1 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
104.8 kDa
Background of RFX1
RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain and the D region found in the C-terminal part of these proteins which facilitates dimerization (1,2). RFX1 is structurally related to regulatory factors X2, X3, X4, and X5 (3). RFX1 is a regulatory factor essential for MHC class II genes expression and can bind to an inverted repeat that is required for expression of hepatitis B virus genes (4). Recent study identifies RFX1 may play a tumor suppressor role in HCC as autophagy mediator (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04392 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RFX1 antibody can be used for detection of RFX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RFX1 in HeLa cell lysate with RFX1 antibody at (A) 1 and (B) 2 μg/ml.
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Immunocytochemistry of RFX1 in HeLa cells with RFX1 at 5 μg/mL.
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Immunofluorescence of RFX1 in Hela cells with RFX1 antibody at 20 μg/mL.
Green: RFX1 Antibody (A04392)
Blue: DAPI staining
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Immunocytochemistry of RFX1 in Hela cells with RFX1 antibody at 5 μg/mL.
Specific Publications For Anti-RFX1 Antibody (A04392)
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