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Product Info Summary
| SKU: | A01284-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-HLF Antibody Picoband®
SKU/Catalog Number
A01284-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HLF Antibody Picoband® catalog # A01284-1. Tested in Flow Cytometry, ICC/IF, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01284-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HLF, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01284-1 is reactive to HLF in Human
Observed Molecular Weight
35 kDa
Calculated molecular weight
33.2 kDa
Background of HLF
Hepatic leukemia factor is a protein that in humans is encoded by the HLF gene. This gene encodes a member of the proline and acidic-rich (PAR) protein family, a subset of the bZIP transcription factors. The encoded protein forms homodimers or heterodimers with other PAR family members and binds sequence-specific promoter elements to activate transcription. Chromosomal translocations fusing portions of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemias.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01284-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human HepG2 whole cell
ICC/IF: HepG2 cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of HLF using anti-HLF antibody (A01284-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLF antigen affinity purified polyclonal antibody (A01284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HLF at approximately 35 kDa. The expected band size for HLF is at 33 kDa.
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IF analysis of HLF using anti-HLF antibody (A01284-1).
HLF was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HLF Antibody (A01284-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow Cytometry analysis of HepG2 cells using anti-HLF antibody (A01284-1).
Overlay histogram showing HepG2 cells stained with A01284-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HLF Antibody (A01284-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-HLF Antibody Picoband® (A01284-1)
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Customer Q&As
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7 Customer Q&As for Anti-HLF Antibody Picoband®
Question
We are currently using anti-HLF antibody A01284-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
The anti-HLF antibody (A01284-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-13
Question
Is there a BSA free version of anti-HLF antibody A01284-1 available?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HLF antibody A01284-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-18
Question
Is a blocking peptide available for product anti-HLF antibody (A01284-1)?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
We do provide the blocking peptide for product anti-HLF antibody (A01284-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-12-19
Question
Is this A01284-1 anti-HLF antibody reactive to the isotypes of HLF?
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
The immunogen of A01284-1 anti-HLF antibody is A synthetic peptide corresponding to a sequence of human HLF (DDLKDDKYWARRRKNNMAAKRSRDARRLKE). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-26
Question
Will A01284-1 anti-HLF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
You can see on the product datasheet, A01284-1 anti-HLF antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-19
Question
Does anti-HLF antibody A01284-1 work on primate WB with hepatoma?
L. Moore
Verified customer
Asked: 2016-03-23
Answer
Our lab technicians have not tested anti-HLF antibody A01284-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-HLF antibody A01284-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate hepatoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-03-23
Question
I was wanting to use your anti-HLF antibody for WB for rat frontal cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat frontal cortex identification?
M. Wu
Verified customer
Asked: 2013-10-16
Answer
You can see on the product datasheet, A01284-1 anti-HLF antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat frontal cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-10-16
