Anti-hnRNP F (HNRNPF) Mouse Monoclonal Antibody [Clone ID: OTI5F5]

Western blot analysis of HNRNPF using anti-HNRNPF antibody (M05806).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SK-OV-3 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human PANC-1 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HNRNPF antigen affinity purified monoclonal antibody (Catalog # M05806) at at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HNRNPF at approximately 46 kDa. The expected band size for HNRNPF is at 46 kDa.

Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and HNRNPF-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-HNRNPF monoclonal antibody M05806

Anti-HNRNPF mouse monoclonal antibody (M05806) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HNRNPF.

Immunofluorescent staining of HepG2 cells using anti-HNRNPF mouse monoclonal antibody (M05806).

Flow cytometric Analysis of Hela cells

Flow cytometric Analysis of Jurkat cells

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNRNPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNRNPF.