Click image to see more details
Product Info Summary
| SKU: | A03243 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Goat |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-HR23A RAD23A Antibody
SKU/Catalog Number
A03243
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-HR23A RAD23A Antibody (Catalog # A03243). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-HR23A RAD23A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03243)
Host
Goat
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 115-140 of human HR23A protein.
Reactive Species
A03243 is reactive to RAD23A in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
39.6 kDa
Background of RAD23A
HR23A (also known as UV excision repair protein RAD23 homolog A) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03243 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:4,000 - 1:16,000
WB: 1:500 - 1:2,000
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to HR23A by western blotting in the appropriate cell lysate or extract.
Validation Images & Assay Conditions
Click image to see more details
Western blot showing Boster's Affinity Purified anti-HR23A antibody detects endogenous human HR23A. Lane 1: HeLa nuclear extract . Lane 2: HeLa . Lane 3: A431 . Lane 4: Jurkat . Lane 5: 293 whole cell lysates . Comparison to a molecular weight marker (at left) indicates a band of ~60 kDa corresponding to HR23A. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using HRP conjugated Rb-a-Goat IgG and chemiluminescence reagent with a 30-min exposure time. Other detection systems will yield similar results.
Specific Publications For Anti-HR23A RAD23A Antibody (A03243)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-HR23A RAD23A Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-HR23A RAD23A Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-HR23A RAD23A Antibody
Question
Is this A03243 anti-HR23A antibody reactive to the isotypes of RAD23A?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
The immunogen of A03243 anti-HR23A antibody is This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 122-135 of human HR23A protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-26
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-HR23A antibody A03243. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-25
Question
Can you help my question with product A03243, anti-HR23A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03243 anti-HR23A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-03
Question
My lab would like to test anti-HR23A antibody A03243 on human liver for research purposes, then I may be interested in using anti-HR23A antibody A03243 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-07
Answer
The products we sell, including anti-HR23A antibody A03243, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-07
Question
We are currently using anti-HR23A antibody A03243 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
M. Zhao
Verified customer
Asked: 2018-10-22
Answer
The anti-HR23A antibody (A03243) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-22
Question
Would anti-HR23A antibody A03243 work on canine WB with muscle of leg?
S. Krishna
Verified customer
Asked: 2016-11-11
Answer
Our lab technicians have not validated anti-HR23A antibody A03243 on canine. You can run a BLAST between canine and the immunogen sequence of anti-HR23A antibody A03243 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine muscle of leg in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-11-11
