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Product Info Summary
|Reactive Species:||Human, Rat|
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Anti-CSB/ERCC6 Antibody Picoband™
Boster Bio Anti-CSB/ERCC6 Antibody Picoband™ catalog # PB10060. Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CSB/ERCC6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10060)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the N-terminus of human CSB (160-205aa QAATSRDINRKLDSVKRQKYNKEQQLKKITAKQKHLQAILGGAEVK).
No cross reactivity with other proteins.
PB10060 is reactive to ERCC6 in Human, Rat
PB10060 is guaranteed for WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: COLO320 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 168 kDa. The expected band size for CSB is at 168 kDa.
Gene/Protein Information For ERCC6 (Source: Uniprot.Org, NCBI)
DNA excision repair protein ERCC-6
SNF2/RAD54 helicase family
ARMD5; ATP-dependent helicase ERCC6; CKN2; Cockayne syndrome group B protein; Cockayne syndrome protein CSB; COFS; COFS1; CSBCockayne syndrome B protein; DNA excision repair protein ERCC-6; EC 3.6.1; EC 3.6.4.-; excision repair cross-complementing rodent repair deficiency, complementationgroup 6; Rad26 homolog; RAD26 ERCC6 ARMD5, CKN2, COFS, COFS1, CSB, CSB-PGBD3, POF11, RAD26, UVSS1 ERCC excision repair 6, chromatin remodeling factor DNA excision repair protein ERCC-6|ERCC6-PGBD3 fusion protein|ATP-dependent helicase ERCC6|Chimeric CSB-PGBD3 protein|Chimeric ERCC6-PGBD3 protein|Cockayne syndrome group B protein|cockayne syndrome protein CSB|excision repair cross-complementation group 6|excision repair cross-complementing rodent repair deficiency, complementation group 6*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ERCC6, check out the ERCC6 Infographic
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In this infographic you will see the following information for ERCC6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for PB10060
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