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Product Info Summary
| SKU: | A14896 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-HSP77/76 Antibody
SKU/Catalog Number
A14896
Size
100μl
Form
Liquid
Description
Boster Bio Anti-HSP77/76 Antibody catalog # A14896. Tested in WB, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP77/76 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14896)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human HSP77/76.
Reactive Species
A14896 is reactive to HSPA7 in Human
Calculated molecular weight
40.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14896 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of K562 cells using HSP77/76 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-HSP77/76 Antibody (A14896)
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Customer Q&As
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6 Customer Q&As for Anti-HSP77/76 Antibody
Question
We are currently using anti-HSP77/76 antibody A14896 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The anti-HSP77/76 antibody (A14896) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
Question
I see that the anti-HSP77/76 antibody A14896 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-03
Question
Is there a BSA free version of anti-HSP77/76 antibody A14896 available?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-HSP77/76 antibody A14896 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-23
Question
Is a blocking peptide available for product anti-HSP77/76 antibody (A14896)?
K. Miller
Verified customer
Asked: 2018-08-10
Answer
We do provide the blocking peptide for product anti-HSP77/76 antibody (A14896). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-08-10
Question
Is this A14896 anti-HSP77/76 antibody reactive to the isotypes of HSPA7?
M. Parker
Verified customer
Asked: 2017-07-04
Answer
The immunogen of A14896 anti-HSP77/76 antibody is Synthesized peptide derived from the Internal region of human HSP77/76. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-04
Question
Will A14896 anti-HSP77/76 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Johnson
Verified customer
Asked: 2016-03-11
Answer
It shows on the product datasheet, A14896 anti-HSP77/76 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-03-11
