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Product Info Summary
| SKU: | M13996 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-HTF9C (TRMT2A) Mouse Monoclonal Antibody [Clone ID: OTI1C3]
SKU/Catalog Number
M13996
Size
100 µl
Description
Boster Bio TRMT2A mouse monoclonal antibody,clone OTI1C3. Catalog# M13996. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-HTF9C (TRMT2A) Mouse Monoclonal Antibody [Clone ID: OTI1C3] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13996)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1C3
Isotype
IgG1
Immunogen
Full length human recombinant protein of human TRMT2A (NP_073564) produced in HEK293T cell.
Reactive Species
M13996 is reactive to TRMT2A in Human, Mouse, Rat
Calculated molecular weight
68.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M13996 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:200~2000
IHC 1:150
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRMT2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRMT2A.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-TRMT2A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TRMT2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRMT2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TRMT2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TRMT2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TRMT2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRMT2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either TRMT2A (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRMT2A antibody (M13996)
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Flow cytometry analysis of U2-OS cells. Red: no-color control; Orange: U2-OS stained with M13996 (1:100) and a secondary with AlexaFlour647; Blue: U2-OS over-expressing the target and stained with the same antibodies. Courtesy: Dr. K. Wei from Stanford University.
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Anti-TRMT2A mouse monoclonal antibody (M13996) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRMT2A.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and TRMT2A-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-TRMT2A monoclonal antibody M13996 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Specific Publications For Anti-HTF9C (TRMT2A) Mouse Monoclonal Antibody [Clone ID: OTI1C3] (M13996)
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