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Product Info Summary
| SKU: | M07106 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-F, WB |
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Product info
Product Name
Anti-Hu CD101 Purified Monoclonal Antibody
SKU/Catalog Number
M07106
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD101 Purified Monoclonal Antibody (Catalog# M07106). Tested in Flow Cytometry, IP, WB, IHC-F application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD101 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07106)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
BB27
Isotype
Mouse IgG1
Immunogen
Human thymic clone B12. The mouse monoclonal antibody BB27 recognizes an extracellular epitope of CD101, a 140 kDa disulfide-bonded homodimeric protein expressed on activated T cells, and some other cell types, such as granulocytes and cells of the monocyte/macropgage lineage.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M07106 is reactive to CD101 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
115.1 kDa
Background of CD101
CD101 is a type I transmembrane glycoprotein, which forms disulfide-linked homodimers. It is expressed on activated T cells, as well as on granulocytes, monocytes, dendritic cells or mucosal T cells. It plays a major role in the activation of T cells by skin dendritic cells. Function of CD101 has not been fully elucidated, but in mice its knock-out results in liver autoimmune disease induced by Novosphingobium aromaticivorans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07106 is guaranteed for Flow Cytometry, IP, IHC-F, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD101 (BB27) purified antibody (concentration in sample 0,56 µg/ml, GAM FITC) and anti-human CD16 (3G8) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).
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Separation of human CD101 positive CD16 negative lymphocytes (red-filled) CD101 negative CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD101 (BB27) purified antibody (concentration in sample 0,56 µg/ml, GAM FITC).
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD101 (BB27) purified antibody (concentration in sample 0,56 µg/ml, GAM FITC).
Specific Publications For Anti-Hu CD101 Purified Monoclonal Antibody (M07106)
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