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Product Info Summary
| SKU: | M00294 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, IHC-F |
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Product info
Product Name
Anti-Hu CD120a Purified TNFRSF1A Monoclonal Antibody
SKU/Catalog Number
M00294
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD120a Purified TNFRSF1A Monoclonal Antibody (Catalog# M00294). Tested in Flow Cytometry, IP, IHC-P, IHC-F application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD120a Purified TNFRSF1A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00294)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
H398
Isotype
Mouse IgG2a
Immunogen
Recombinant full length human CD120a. The mouse monoclonal antibody H398 recognizes the extracellular domain of CD120a, a 55 kDa receptor for tumor necrosis factor. The antibody blocks biological activity of both natural and recombinant human TNF alpha and TNF beta.
Reactive Species
M00294 is reactive to TNFRSF1A in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
50.5 kDa
Background of TNFRSF1A
CD120a / TNF R1, also known as TNFR55 or TNFRSF1A, is a 55 kDa receptor for tumor necrosis factor alpha and it is expressed in most tissues. By binding its trimeric ligand the CD120a protein forms trimers and the conformation change leads to dissociation of the inhibitory factor SODD from its intracellular death domain and in formation of signaling platform. CD120a can mediate apoptosis, and function as a regulator of inflammation. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00294 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Separation of human monocytes (red-filled) from CD120a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD120a (H398) purified antibody (concentration in sample 3 µg/ml) GAM APC.
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD120a (H398) purified antibody (concentration in sample 3 µg/ml) GAM APC.
Specific Publications For Anti-Hu CD120a Purified TNFRSF1A Monoclonal Antibody (M00294)
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