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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human Matrilin-3 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Matrilin-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MATN3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Matrilin-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2022)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: D29-R486
93.7 pg/ml - 6,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK2022 is reactive to MATN3 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK2022 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MATN3
Matrilin-3 is a protein that in humans is encoded by the MATN3 gene. This gene is mapped to 2p24.1. This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains two von Willebrand factor A domains; it is present in the cartilage extracellular matrix and has a role in the development and homeostasis of cartilage and bone. Mutations in this gene result in multiple epiphyseal dysplasia.
|EK2022-CAP||Anti-Human MATN3 Pre-coated 96-well strip microplate||1|
|EK2022-ST||Human MATN3 Standard||2 vials, 10 ng/tube|
|EK2022-DA||Human MATN3 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Matrilin-3 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MATN3, Dilution ratio of 1:1, concentration in serum is around 250pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Matrilin-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of MATN3 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For MATN3 (Source: Uniprot.Org, NCBI)
DIPOA; EDM5; HOA; matrilin 3; matrilin-3; OADIP; OS2 MATN3 DIPOA, EDM5, HOA, OADIP, OS2, SEMDBCD matrilin 3 matrilin-3*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MATN3, check out the MATN3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MATN3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK2022
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