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Human TNFsR I ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFsR I. 96wells/kit, with removable strips.
Human TNFsR I ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0528 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | For quantitative detection of human TNFsR?in cell culture supernates, serum, plasma( heparin, EDTA, citrate) and urine. |
| Sample Volume: | 100μl per well |
| Sensitivity: | <1pg/ml |
| Assay Range: | 7.8pg/ml-500pg/ml |
Overview
| Product Name | Human TNFsR I ELISA Kit PicoKine™
See all TNFRSF1A primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0528 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Human Tnf Ri/TNFRSF1A with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: For quantitative detection of human TNFsR?in cell culture supernates, serum, plasma( heparin, EDTA, citrate) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Tnf Ri/TNFRSF1A tissue specificity: |
| Cite This Product | Human TNFsR I ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0528) |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | For quantitative detection of human TNFsR?in cell culture supernates, serum, plasma( heparin, EDTA, citrate) and urine. |
| Sensitivity | <1pg/ml |
| Assay Range | 7.8pg/ml-500pg/ml |
| Immunogen | Expression system for standard: NSO, Immunogen sequence: L30-T211 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P19438 |
|---|---|
| Gene Name | TNFRSF1A |
| Protein Name | Tumor necrosis factor receptor superfamily member 1A |
| Subcellular Localization | Cell membrane ; Single-pass type I membrane protein . Golgi apparatus membrane ; Single- pass type I membrane protein . Secreted . A secreted form is produced through proteolytic processing. |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Human TNFsR I ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0528-CAP | 96-well plate precoated with anti-Human TNFRSF1A antibody | 1 |
| EK0528-ST | lyophilized recombinant Human TNFRSF1A standard | 10ng/tube |
| EK0528-DA | biotinylated anti-Human TNFRSF1A antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0528 Human TNFsR I ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.010 | 0.072 | 0.176 | 0.348 | 0.576 | 1.135 | 1.829 | 2.326 |
We measured random samples of Human TNFsR I ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 10 | 54 | 219 | 10 | 59 | 203 |
| Standard deviation | 0.55 | 3.99 | 13.14 | 0.58 | 4.48 | 13.39 |
| CV(%) | 5.5% | 7.4% | 6% | 5.8% | 7.6% | 6.6% |
We measured the performance consistency of Human TNFsR I ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 10 | 10 | 9 | 9 | 9 | 0.5 | 5.5% |
| Sample 2 | 54 | 52 | 56 | 55 | 54 | 1.47 | 2.7% |
| Sample 3 | 219 | 194 | 207 | 198 | 204 | 9.6 | 4.7% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Questions and answers from customers related to EK0528 Human TNFsR I ELISA Kit PicoKine™
1 Related Questions
Question
Q: Can EK0528 be used for cell lysate samples? <br>Keywords: sample types
Verified Customer
Asked: 0000-00-00
Answer
A: EK0528 is used to detect soluble fragment. Our lab hasn't tested EK0528 with any cell lysate, so we do not recommend using EK0528 to test cell lysate samples.
Boster Scientific Support
Answered: 0000-00-00
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Shipping Information
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
Contact Information
| [email protected] | |
| (888) 466-3604 |
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