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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine.|
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Human TNFsR I ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human TNFsR I ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF1A in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TNFsR I ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0528)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L30-T211
7.8 pg/ml - 500 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0528 is reactive to TNFRSF1A in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine.
EK0528 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0528-CAP||Anti-Human TNFRSF1A Pre-coated 96-well strip microplate||1|
|EK0528-ST||Human TNFRSF1A Standard||2 vials, 10 ng/tube|
|EK0528-DA||Human TNFRSF1A Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human TNFsR I PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TNF RI/TNFRSF1A, Dilution ratio of 1:20, concentration in serum is around 3.2ng/ml.
Some articles we found to cite concentrations of TNF RI/TNFRSF1A in samples: 24803721 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TNFsR I ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of TNF RI/TNFRSF1A in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For TNFRSF1A (Source: Uniprot.Org, NCBI)
Tumor necrosis factor receptor superfamily member 1A
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor TNFRSF1A CD120a, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60 TNF receptor superfamily member 1A tumor necrosis factor receptor superfamily member 1A|TNF-R1|TNF-RI|TNFR-I|tumor necrosis factor binding protein 1|tumor necrosis factor receptor type 1|tumor necrosis factor-alpha receptor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TNFRSF1A, check out the TNFRSF1A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0528 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Determination Levels of Tumor Necrosis Factor Receptor and Tolk like Receptor-4 and Bacterial Vaginosis in Women with Recurrent Spontaneous Abortion. Sumaya Yahya Abed Obais,Shaimaa Jassim Alsultany
EK0528 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
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