Human TNFsR I ELISA Kit PicoKine™

Human TNFsR I ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF1A in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0528
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine.

Product Name

Human TNFsR I ELISA Kit PicoKine™

See all TNF RI/TNFRSF1A products

SKU/Catalog Number

EK0528

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TNFsR I ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF1A in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TNFsR I ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0528)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L30-T211

Sensitivity

<1 pg/ml

Assay Range

7.8 pg/ml - 500 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0528 is reactive to TNFRSF1A in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine.

Application Guarantee

EK0528 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0528-CAP Anti-Human TNFRSF1A Pre-coated 96-well strip microplate 1
EK0528-ST Human TNFRSF1A Standard 2 vials, 10 ng/tube
EK0528-DA Human TNFRSF1A Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)07.815.631.362.5125250500
O.D.0.0100.0720.1760.3480.5761.1351.8292.326

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TNF RI/TNFRSF1A, Dilution ratio of 1:20, concentration in serum is around 3.2ng/ml.

Some articles we found to cite concentrations of TNF RI/TNFRSF1A in samples: 24803721 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TNFsR I ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)10542191059203
Standard deviation0.553.9913.140.584.4813.39
CV (%)5.5%7.4%6%5.8%7.6%6.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TNF RI/TNFRSF1A in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 110109990.55.5%
Sample 254525655541.472.7%
Sample 32191942071982049.64.7%
*number of samples for each test n=16.

Gene/Protein Information For TNFRSF1A (Source: Uniprot.Org, NCBI)

Uniprot ID

P19438

Gene ID

7132

Gene Name

TNFRSF1A

Full Name

Tumor necrosis factor receptor superfamily member 1A

Weight

50.495kDa

Alternative Names

CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor TNFRSF1A CD120a, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60 TNF receptor superfamily member 1A tumor necrosis factor receptor superfamily member 1A|TNF-R1|TNF-RI|TNFR-I|tumor necrosis factor binding protein 1|tumor necrosis factor receptor type 1|tumor necrosis factor-alpha receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNFRSF1A, check out the TNFRSF1A Infographic

TNFRSF1A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNFRSF1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0528 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Determination Levels of Tumor Necrosis Factor Receptor and Tolk like Receptor-4 and Bacterial Vaginosis in Women with Recurrent Spontaneous Abortion. Sumaya Yahya Abed Obais,Shaimaa Jassim Alsultany
Species: Human
EK0528 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

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9 Customer Q&As for Human TNFsR I ELISA Kit PicoKine™

Question

I have some inquiry regarding your product Human TNFsR I ELISA Kit PicoKine. Do you have any records about its endotoxin level? What protocol used in order to determine the antibody neutralization activity (it's probably proliferation of PBMC but I need the protocol). Is it azide free? Please advise.

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

The Human TNFsR I ELISA Kit PicoKine endotoxin level is <0.5EU/mg antibody and yes it is azide free. Costumers needs to optimize the conditions, as the culture condition is different. The assay was performed on human peripheral blood mononuclear cells, incubated with 10 ng/ml TNSF4 for 5-6 days in serum-containing media. Other cells were incubated with TNSF4 that has been first incubated with 5-15ng/ml at 37C for one hour before adding to the medium.

Boster Scientific Support

Answered: 2020-04-06

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-12-10

Question

Can EK0528 be used for cell lysate samples? <br>Keywords: sample types

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

EK0528 is used to detect soluble fragment. Our lab hasn't tested EK0528 with any cell lysate, so we do not recommend using EK0528 to test cell lysate samples.

Boster Scientific Support

Answered: 2019-10-08

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-08-16

Question

Q: What is the optimal O.D. value for TNF RI ELISA kit? I performed your TNF RI ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TNF RI even though the O.D. values are not very high?

C. Williams

Verified customer

Asked: 2019-08-05

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TNF RI ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of TNF RI that can be declared/interpreted as positive by the above standard is 1pg/ml.

Boster Scientific Support

Answered: 2019-08-05

Question

Q: how do I analyze ELISA data? I have performed TNF RI level in urine.

G. Anderson

Verified customer

Asked: 2019-07-06

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-07-06

Question

Q: if the enzyme conjugated TNF RI antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TNF RI antigen?

Z. Collins

Verified customer

Asked: 2018-07-19

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-07-19

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-03-12

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-03-12

Question

Q: can I use heparin plasma as samples in Human TNF RI Picokine® ELISA Kit (Catalog # EK0528)?

Verified Customer

Verified customer

Asked: 2015-01-26

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of TNF RI causing degradation of its protein structure. TNF RI may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TNF RI ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2015-01-26

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