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Product Info Summary
| SKU: | EK0528 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. |
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Product info
Product Name
Human TNFsR I ELISA Kit PicoKine®
View all TNF RI/TNFRSF1A ELISA kits
SKU/Catalog Number
EK0528
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TNFsR I ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF1A in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TNFsR I ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0528)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L30-T211
Sensitivity
<1 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0528 is reactive to TNFRSF1A in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine.
Application Guarantee
EK0528 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TNF RI/TNFRSF1A
TNFsRI (also known as Solube tumor necrosis factor receptor 1) is a cytokine receptor that binds tumor necrosis factors (TNF). In contrast, the p60 (TNFRSF1A) and p80 (TNFRSF1B) TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain (PLAD), in the absence of ligand. Deletion of the PLAD results in monomeric presentation of p60 or p80. Flow cytometric analysis showed that efficient TNFA binding depends on receptor self-assembly. They also found that other members of the TNF receptor superfamily, including the extracellular domains of TRAIL (TNFRSF10A), CD40, and FAS (TNFRSF6), all self-associate but do not interact with heterologous receptors. By Southern blot analysis of human/Chinese hamster somatic cell hybrid DNA, the TNFR1 gene was mapped to 12pter-cen. And by nonradioactive in situ hybridization that the type 1 receptor (the p55 TNF receptor) is encoded by a gene located on chromosome 12p13.2.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0528-CAP | Anti-Human TNFRSF1A Pre-coated 96-well strip microplate | 1 |
| EK0528-ST | Human TNFRSF1A Standard | 2 vials, 10 ng/tube |
| EK0528-DA | Human TNFRSF1A Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.010 | 0.072 | 0.176 | 0.348 | 0.576 | 1.135 | 1.829 | 2.326 |
Data Example Images
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Human TNFsR I PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TNF RI/TNFRSF1A, Dilution ratio of 1:20, concentration in serum is around 3.2ng/ml.
Some articles we found to cite concentrations of TNF RI/TNFRSF1A in samples: 24803721 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TNFsR I ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 10 | 54 | 219 | 10 | 59 | 203 |
| Standard deviation | 0.55 | 3.99 | 13.14 | 0.58 | 4.48 | 13.39 |
| CV (%) | 5.5% | 7.4% | 6% | 5.8% | 7.6% | 6.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TNF RI/TNFRSF1A in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 10 | 10 | 9 | 9 | 9 | 0.5 | 5.5% |
| Sample 2 | 54 | 52 | 56 | 55 | 54 | 1.47 | 2.7% |
| Sample 3 | 219 | 194 | 207 | 198 | 204 | 9.6 | 4.7% |
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF1A (Source: Uniprot.Org, NCBI)
Uniprot ID
P19438
Gene ID
7132
Gene Name
TNFRSF1A
Full Name
Tumor necrosis factor receptor superfamily member 1A
Weight
50.495kDa
Alternative Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor TNFRSF1A CD120a, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60 TNF receptor superfamily member 1A tumor necrosis factor receptor superfamily member 1A|TNF-R1|TNF-RI|TNFR-I|tumor necrosis factor binding protein 1|tumor necrosis factor receptor type 1|tumor necrosis factor-alpha receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFRSF1A, check out the TNFRSF1A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human TNFsR I ELISA Kit PicoKine® (EK0528)
EK0528 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Endothelial damage is aggravated in acute GvHD and could predict its development
Determination Levels of Tumor Necrosis Factor Receptor and Tolk like Receptor-4 and Bacterial Vaginosis in Women with Recurrent Spontaneous Abortion. Sumaya Yahya Abed Obais,Shaimaa Jassim Alsultany
Species: Human
EK0528 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human TNFsR I ELISA Kit PicoKine®
Question
I have some inquiry regarding your product Human TNFsR I ELISA Kit PicoKine. Do you have any records about its endotoxin level? What protocol used in order to determine the antibody neutralization activity (it's probably proliferation of PBMC but I need the protocol). Is it azide free? Please advise.
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
The Human TNFsR I ELISA Kit PicoKine endotoxin level is <0.5EU/mg antibody and yes it is azide free. Costumers needs to optimize the conditions, as the culture condition is different. The assay was performed on human peripheral blood mononuclear cells, incubated with 10 ng/ml TNSF4 for 5-6 days in serum-containing media. Other cells were incubated with TNSF4 that has been first incubated with 5-15ng/ml at 37C for one hour before adding to the medium.
Boster Scientific Support
Answered: 2020-04-06
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-10
Question
Can EK0528 be used for cell lysate samples? <br>Keywords: sample types
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
EK0528 is used to detect soluble fragment. Our lab hasn't tested EK0528 with any cell lysate, so we do not recommend using EK0528 to test cell lysate samples.
Boster Scientific Support
Answered: 2019-10-08
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-08-16
Question
Q: What is the optimal O.D. value for TNF RI ELISA kit? I performed your TNF RI ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TNF RI even though the O.D. values are not very high?
C. Williams
Verified customer
Asked: 2019-08-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TNF RI ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of TNF RI that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2019-08-05
Question
Q: how do I analyze ELISA data? I have performed TNF RI level in urine.
G. Anderson
Verified customer
Asked: 2019-07-06
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-07-06
Question
Q: if the enzyme conjugated TNF RI antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TNF RI antigen?
Z. Collins
Verified customer
Asked: 2018-07-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-07-19
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-03-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-03-12
Question
Q: can I use heparin plasma as samples in Human TNF RI Picokine® ELISA Kit (Catalog # EK0528)?
Verified Customer
Verified customer
Asked: 2015-01-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of TNF RI causing degradation of its protein structure. TNF RI may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TNF RI ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-01-26
