Overview
| Product Name |
Human TNFsR I ELISA Kit PicoKine™
See more TNF RI/TNFRSF1A products |
| Catalog# |
EK0528 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Human Tnf Ri/TNFRSF1A with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: For quantitative detection of human TNFsR?in cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Tnf Ri/TNFRSF1A tissue specificity: |
| Cite This Product |
Human TNFsR I ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0528)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, serum, plasma ( heparin, EDTA, citrate) and urine. |
| Sensitivity |
<1pg/ml |
| Assay Range |
7.8pg/ml-500pg/ml |
| Immunogen/Standard |
Expression system for standard: NSO, Immunogen sequence: L30-T211 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For TNFRSF1A (Source: Uniprot.org, NCBI)
| Uniprot Id | P19438 |
|---|
| NCBI Gene Id | 7132 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | TNFRSF1A |
|---|
| Protein Name | Tumor necrosis factor receptor superfamily member 1A |
|---|
| Alternative Names | TNF RI/TNFRSF1A|CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor |
|---|
| Molecular Weight | 50495 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TNFRSF1A, check out the TNFRSF1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for TNFRSF1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the TNFRSF1A infographic
Kit Components And Assay QC Data Details Of Human TNFsR I ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0528-CAP |
Anti-Human TNFRSF1A Pre-coated 96-well strip microplate | 1 |
| EK0528-ST | Human TNFRSF1A Standard | 2 vials, 10ng/tube |
| EK0528-DA | Human TNFRSF1A Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| - | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0528 Human TNFsR I ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.010 | 0.072 | 0.176 | 0.348 | 0.576 | 1.135 | 1.829 | 2.326 |
We measured random samples of Human TNFsR I ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 10 | 54 | 219 | 10 | 59 | 203 |
| Standard deviation | 0.55 | 3.99 | 13.14 | 0.58 | 4.48 | 13.39 |
| CV(%) | 5.5% | 7.4% | 6% | 5.8% | 7.6% | 6.6% |
We measured the performance consistency of Human TNFsR I ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 10 | 10 | 9 | 9 | 9 | 0.5 | 5.5% |
| Sample 2 | 54 | 52 | 56 | 55 | 54 | 1.47 | 2.7% |
| Sample 3 | 219 | 194 | 207 | 198 | 204 | 9.6 | 4.7% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 0
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Questions and answers from customers related to EK0528 Human TNFsR I ELISA Kit PicoKine™
9 Related Questions
Question
I have some inquiry regarding your product Human TNFsR I ELISA Kit PicoKine. Do you have any records about its endotoxin level? What protocol used in order to determine the antibody neutralization activity (it's probably proliferation of PBMC but I need the protocol). Is it azide free? Please advise.
Verified Customer
Asked: 2020-04-06
Answer
The Human TNFsR I ELISA Kit PicoKine endotoxin level is <0.5EU/mg antibody and yes it is azide free. Costumers needs to optimize the conditions, as the culture condition is different. The assay was performed on human peripheral blood mononuclear cells, incubated with 10 ng/ml TNSF4 for 5-6 days in serum-containing media. Other cells were incubated with TNSF4 that has been first incubated with 5-15ng/ml at 37C for one hour before adding to the medium.
Boster Scientific Support
Answered: 2020-04-06
Question
Can EK0528 be used for cell lysate samples?
<br>Keywords: sample types
Verified Customer
Asked: 2019-10-08
Answer
EK0528 is used to detect soluble fragment. Our lab hasn't tested EK0528 with any cell lysate, so we do not recommend using EK0528 to test cell lysate samples.
Boster Scientific Support
Answered: 2019-10-08
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2019-12-10
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-10
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2019-08-16
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-08-16
Question
Q: What is the optimal O.D. value for TNF RI ELISA kit? I performed your TNF RI ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TNF RI even though the O.D. values are not very high?
C. Williams
Asked: 2019-08-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TNF RI ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of TNF RI that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2019-08-05
Question
Q: how do I analyze ELISA data? I have performed TNF RI level in urine.
G. Anderson
Asked: 2019-07-06
Boster Scientific Support
Answered: 2019-07-06
Question
Q: if the enzyme conjugated TNF RI antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TNF RI antigen?
Z. Collins
Asked: 2018-07-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-07-19
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Asked: 2018-03-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-03-12
Question
Q: can I use heparin plasma as samples in Human TNF RI Picokine® ELISA Kit (Catalog # EK0528)?
Verified Customer
Asked: 2015-01-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of TNF RI causing degradation of its protein structure. TNF RI may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TNF RI ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-01-26
