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Product Info Summary
| SKU: | M05615 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, ICC |
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Product info
Product Name
Anti-Hu CD203c Purified ENPP3 Monoclonal Antibody
SKU/Catalog Number
M05615
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD203c Purified ENPP3 Monoclonal Antibody (Catalog# M05615). Tested in Flow Cytometry, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD203c Purified ENPP3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05615)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
NP4D6
Isotype
Mouse IgG1
Immunogen
HEK-293 cells transfected with human CD203c. The mouse monoclonal antibody NP4D6 reacts with an extracellular epitope of CD203c, a transmembrane ectoenzyme expressed on basophils and mast cells, and overexpressed upon their activation.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M05615 is reactive to ENPP3 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
100.1 kDa
Background of ENPP3
CD203c, also known as ENPP-3, is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05615 is guaranteed for Flow Cytometry, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml. Extracellular and intracellular staining.
Validation Images & Assay Conditions
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Separation of CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of IgE stimulated human peripheral whole blood using anti-human CD203c (NP4D6) purified antibody (concentration in sample 2 µg/ml, GAM APC).
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Flow cytometry surface staining pattern of IgE stimulated human peripheral whole blood stained using anti-human CD203c (NP4D6) purified antibody (concentration in sample 2 µg/ml, GAM APC).
Specific Publications For Anti-Hu CD203c Purified ENPP3 Monoclonal Antibody (M05615)
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