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Product Info Summary
| SKU: | A05922 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-HVCN1 Antibody
SKU/Catalog Number
A05922
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-HVCN1 Antibody (Catalog # A05922). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
HVCN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-HVCN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05922)
Host
Rabbit
Contents
HVCN1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
HVCN1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human HVCN1. The immunogen is located within the last 50 amino acids of HVCN1.
Reactive Species
A05922 is reactive to HVCN1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
31.7 kDa
Background of HVCN1
HVCN1 (hydrogen voltage-gated channel 1), a voltage gated proton channel protein that is highly expressed in immune tissues, mediates the proton conductances required by phagocytic leukocytes for the oxidative burst that underlies microbial killing. HVCN1 moderates the voltage-dependent proton permeability of excitable membranes by allowing the flow of protons in accordance to their electrochemical gradient. HVCN1 is sensitive to zinc ions, and can be inhibited by them. HVCN1 may represent a new therapeutic target for B cell malignancies on continued signaling via the B cell antigen receptor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05922 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
HVCN1 antibody can be used for detection of HVCN1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of HVCN1 in (A) human spleen and (B) mouse spleen tissue lysates with HVCN1 antibody at 1 μg/mL.
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Immunohistochemistry of HVCN1 in rat spleen tissue with HVCN1 antibody at 5 μg/mL.
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Immunofluorescence of HVCN1 in Rat Spleen cells with HVCN1 antibody at 20 μg/mL.
Specific Publications For Anti-HVCN1 Antibody (A05922)
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