Anti-Hu CD7 Purified Monoclonal Antibody

Product Name

Anti-Hu CD7 Purified Monoclonal Antibody

View all CD7 Antibodies

SKU/Catalog Number

M01974-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD7 Purified Monoclonal Antibody (Catalog# M01974-1). Tested in Flow Cytometry, IP, WB, IHC-P application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD7 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01974-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MEM-186

Isotype

Mouse IgG1

Immunogen

Human acute myelogenous leukaemia cell line KG-1. The antibody MEM-186 reacts with an extracellular epitope of CD7, a 40 kD type I transmembrane glycoprotein expressed on peripheral blood T lymphocytes, NK-cells, hematopoietic progenitors, monocytes (weakly) and also on acute lymphocytic leukemia.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M01974-1 is reactive to CD7 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

25.4 kDa

Background of CD7

CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01974-1 is guaranteed for Flow Cytometry, IP, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (paraffin sections): 5 μg/ml; positive tissue: spleen.
Flow cytometry: 2 μg/ml.
Western blotting: Non-reducing conditions.

Validation Images & Assay Conditions

See full target information CD7

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