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Product Info Summary
| SKU: | M04575-4 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, ICC |
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Product info
Product Name
Anti-Human Gamma Heavy Chain IGHG1 Rabbit Monoclonal Antibody, Clone#RM116
SKU/Catalog Number
M04575-4
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human Gamma Heavy Chain IGHG1 Rabbit Monoclonal Antibody, Clone#RM116 (Catalog # M04575-4). Tested in ELISA, ICC, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human Gamma Heavy Chain IGHG1 Rabbit Monoclonal Antibody, Clone#RM116 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04575-4)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM116
Isotype
Rabbit IgG
Immunogen
Human IgG
Cross-reactivity
This antibody reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including γ1, γ2, γ3, and γ4. No cross-reactivity with other human heavy chains, mouse IgG, rat IgG or goat IgG
Reactive Species
M04575-4 is reactive to IGHG1 in Human
Calculated molecular weight
36.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04575-4 is guaranteed for ELISA, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection)
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Validation Images & Assay Conditions
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IHC result
Immunohistochemistry of human tonsil using anti-gamma heavy chain antibody RM116.
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ELISA result showing specificity
ELISA of human immunoglobulins shows RM116 reacted to the y1, y2, y3, y4 heavy chain of human IgGs, and the Fc of human IgG. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
A titer ELISA using RM116. The plate was coated with different amounts of human IgG1. A serial dilution of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
Cross-activity data of anti-human IgG antibodies against monkey IgG. ELISA plate was coated with 0.1ug/well (2ug/mL, 50uL/well) of Rhesus monkey IgG (protein A purified from serum). A serial dilution of anti-human IgG (RM116), anti-human IgG1 (RM117), anti-human IgG2 (RM118), anti-human IgG3 RM119), (anti-human IgG4 (RM120), anti-human kappa (RM126), and anti-human lambda (RM127) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
Cross-activity data of anti-human IgG antibodies against monkey IgG. ELISA plate was coated with 0.1ug/well (2ug/mL, 50uL/well) of Rhesus monkey IgG (protein A purified from serum). A serial dilution of anti-human IgG (RM116), anti-human IgG1 (RM117), anti-human IgG2 (RM118), anti-human IgG3 RM119), (anti-human IgG4 (RM120), anti-human kappa (RM126), and anti-human lambda (RM127) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Click image to see more details
ELISA result showing specificity
Cross-activity data of anti-human IgG antibodies against monkey IgG. ELISA plate was coated with 0.1ug/well (2ug/mL, 50uL/well) of Rhesus monkey IgG (protein A purified from serum). A serial dilution of anti-human IgG (RM116), anti-human IgG1 (RM117), anti-human IgG2 (RM118), anti-human IgG3 RM119), (anti-human IgG4 (RM120), anti-human kappa (RM126), and anti-human lambda (RM127) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Specific Publications For Anti-Human Gamma Heavy Chain IGHG1 Rabbit Monoclonal Antibody, Clone#RM116 (M04575-4)
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