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Product Info Summary
| SKU: | M05470-4 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, ICC |
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Product info
Product Name
Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody, Clone#RM129
SKU/Catalog Number
M05470-4
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody, Clone#RM129 (Catalog # M05470-4). Tested in ELISA, ICC, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody, Clone#RM129 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05470-4)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM129
Isotype
Rabbit IgG
Immunogen
Human IgG
Cross-reactivity
This antibody reacts to both kappa and lambda light chains of human immunoglobulins. The antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Reactive Species
M05470-4 is reactive to IGKC in Human
Calculated molecular weight
11.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05470-4 is guaranteed for ELISA, Flow Cytometry, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 0.1 ug/mL – 0.5 ug/mL
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL
Flow Cytometry (FC): 0.5ug/mL-2ug/mL.
Validation Images & Assay Conditions
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ELISA result showing specificity
ELISA shows that clone RM129 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM129 and a monkey IgG reacting antibody (positive control) was used as the detection antibodies.
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ELISA result showing specificity
ELISA shows that clone RM129 reacts only to the kappa and lambda light chains in all human immunoglobulins, and does not react to mouse IgG, rat IgG, or goat IgG.
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ELISA result showing specificity
A titer ELISA using RM129. The plate was coated with different amounts of human IgG4. A serial dilution of RM129 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
A titer ELISA using RM129. The plate was coated with different amounts of human IgG3. A serial dilution of RM129 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Specific Publications For Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody, Clone#RM129 (M05470-4)
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Customer Q&As
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5 Customer Q&As for Anti-Human Ig Light Chains IGKC Rabbit Monoclonal Antibody, Clone#RM129
Question
We are currently using anti-Human Ig Light Chains Rabbit Monoclonal antibody, Clone#RM129 M05470-4 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-Human Ig Light Chains Rabbit Monoclonal antibody, Clone#RM129 (M05470-4) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04
Question
Does anti-Human Ig Light Chains Rabbit Monoclonal antibody, Clone#RM129 M05470-4 work on canine ELISA with abdominal adipose tissue?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
Our lab technicians have not tested anti-Human Ig Light Chains Rabbit Monoclonal antibody, Clone#RM129 M05470-4 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Human Ig Light Chains Rabbit Monoclonal antibody, Clone#RM129 M05470-4 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine abdominal adipose tissue in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-26
Question
See attached the WB image, lot number and protocol we used for liver using anti-Human Ig Light Chains Rabbit Monoclonal antibody, Clone#RM129 M05470-4. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-20
Question
I have a question about product M05470-4, anti-Human Ig Light Chains Rabbit Monoclonal antibody, Clone#RM129. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05470-4 anti-Human Ig Light Chains Rabbit Monoclonal antibody, Clone#RM129, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-06
Question
Does M05470-4 anti-Human Ig Light Chains Rabbit Monoclonal antibody, Clone#RM129 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Williams
Verified customer
Asked: 2016-06-24
Answer
It shows on the product datasheet, M05470-4 anti-Human Ig Light Chains Rabbit Monoclonal antibody, Clone#RM129 as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-06-24
