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Product Info Summary
| SKU: | PA1734 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Toll-like receptor 8 TLR8 Antibody Picoband®
SKU/Catalog Number
PA1734
BA3805-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Toll-like receptor 8 TLR8 Antibody catalog # PA1734. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Toll-like receptor 8 TLR8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1734)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat TLR8, different from the related mouse sequence by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1734 is reactive to Tlr8 in Rat
Observed Molecular Weight
150 kDa
Background of Tlr8
TLR8 (Toll-like receptor 8) is a protein that in humans is encoded by the TLR8 gene. TLR8 has also been designated as CD288 (cluster of differentiation 288). The TLR8 gene is mapped to Xp22.3-p22.2 by Chuang and Ulevitch (2000) and Du et al. (2000). The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X. TLR8 recognises G-rich oligonucleotides.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1734 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat
Positive Control
WB: rat liver tissue
Validation Images & Assay Conditions
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Western blot analysis of TLR8 using anti-TLR8 antibody (PA1734).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR8 antigen affinity purified polyclonal antibody (Catalog # PA1734) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR8 at approximately 150 kDa. The expected band size for TLR8 is at 120 kDa.
Specific Publications For Anti-Toll-like receptor 8 TLR8 Antibody Picoband® (PA1734)
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4 Customer Q&As for Anti-Toll-like receptor 8 TLR8 Antibody Picoband®
Question
My colleagues were well pleased with the WB result of your anti-TLR8 antibody. However we have observed positive staining in placenta membrane using this antibody. Is that expected? Could you tell me where is TLR8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
From literature, placenta does express TLR8. Generally TLR8 expresses in membrane. Regarding which tissues have TLR8 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Placenta, Pubmed ID: 11022119, 11022120, 18810425
Boster Scientific Support
Answered: 2020-02-10
Question
Our lab want to know about using your anti-TLR8 antibody for defense response to virus studies. Has this antibody been tested with western blotting on u87 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
Thanks for your inquiry. This PA1734 anti-TLR8 antibody is validated on u87 whole cell lysate. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-06
Question
We have seen staining in mouse placenta. What should we do? Is anti-TLR8 antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
From what I have seen in literature placenta does express TLR8. From what I have seen in Uniprot.org, TLR8 is expressed in blood, placenta, liver, among other tissues. Regarding which tissues have TLR8 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Placenta, Pubmed ID: 11022119, 11022120, 18810425
Boster Scientific Support
Answered: 2019-09-03
Question
We are currently using anti-TLR8 antibody PA1734 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
The anti-TLR8 antibody (PA1734) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-13
