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Product Info Summary
| SKU: | M07514-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, ICC |
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Product info
Product Name
Anti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124
SKU/Catalog Number
M07514-2
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124 (Catalog # M07514-2). Tested in ELISA, ICC, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07514-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM124
Isotype
Rabbit IgG
Immunogen
Human IgA
Cross-reactivity
This antibody reacts to human IgA1, and very slightly cross reacts with IgA2. No cross-reactivity with human IgG, IgM, IgD, or IgE.
Reactive Species
M07514-2 is reactive to IGHA1 in Human
Calculated molecular weight
37.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07514-2 is guaranteed for ELISA, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection)
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.1ug/mL-1ug/mL.
Validation Images & Assay Conditions
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IHC result
Immunohistochemistry of human tonsil tissue using anti-human IgA1 antibody RM124
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ELISA result showing specificity
Sandwich ELISA using RM124 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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ELISA result showing specificity
Sandwich ELISA using RM124 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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ELISA result showing specificity
ELISA of human immunoglobulins shows RM124 only reacted to human IgA1. Very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of RM124 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
A titer ELISA using RM124. The plate was coated with different amounts of human IgA1. A serial dilution of RM124 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Specific Publications For Anti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124 (M07514-2)
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1 Customer Q&As for Anti-Human IgA1 IGHA1 Rabbit Monoclonal Antibody, Clone#RM124
Question
We are currently using anti-Human IgA1 Rabbit Monoclonal antibody, Clone#RM124 M07514-2 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
The anti-Human IgA1 Rabbit Monoclonal antibody, Clone#RM124 (M07514-2) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-11
