Product Info Summary
SKU: | A07747 |
---|---|
Size: | 100uL |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-ALG6 Antibody
SKU/Catalog Number
A07747
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-ALG6 Antibody (Catalog # A07747). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-ALG6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07747)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of human ALG6 protein, conjugated to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07747 is reactive to ALG6 in Human
Applications
A07747 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
58.121kDa
Background of ALG6
Anti-ALG6 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase is an enzyme that is encoded by the ALG6 gene in humans. This gene encodes ALG6/ALG8 member of glucosyltransferase family. The encoded protein catalyzes the addition of the first glucose residue to the growing lipid-linked oligosaccharide precursor of N-linked glycosylation. Mutations in this gene are associated with congenital disorders of glycosylation type. Anti-ALG6 is ideal for researchers interested in Infectious disease, especially HIV and hepatitis virus infections. Anti-ALG6 antibody is applicable to methods for diagnosis and, in particular, to methods for diagnosing infections using biomarkers targeting exosomes secreted in bodily fluids.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:45,000
IHC: 4µg/mL
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ALG6 expression in HELA whole cell lysates (lane 1), MOLT4 Whole Cell lysate (lane 2) and K-562 Whole Cell lysate (lane 3). ALG6 at 58KD was detected using rabbit anti-ALG6 Antigen Affinity purified polyclonal antibody (Catalog # A07747) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Click image to see more details
ALG6 was detected in paraffin-embedded sections of the squamous epithelium of the uterine cervix as well as in inflammatory elements diffused in the stroma of human cervical cancer tissue at 20x and 40x (B & C) using rabbit anti-ALG6 Antigen Affinity purified polyclonal antibody (Catalog # A07747) at 4 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Protein Target Info & Infographic
Gene/Protein Information For ALG6 (Source: Uniprot.org, NCBI)
Gene Name
ALG6
Full Name
Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
Weight
58.121kDa
Superfamily
ALG6/ALG8 glucosyltransferase family
Alternative Names
alpha-1,3-glucosyltransferase); asparagine-linked glycosylation 6 homolog (S. cerevisiae; asparagine-linked glycosylation 6 homolog (yeast; asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S.cerevisiae); Asparagine-linked glycosylation protein 6 homolog; CDG1C; dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase; Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase; dolichyl-P-Glc:Man9GlcNAc2-PP-dolichylglucosyltransferase; EC 2.4.1; EC 2.4.1.-; Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase ALG6 CDG1C ALG6 alpha-1,3-glucosyltransferase dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase|Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase|asparagine-linked glycosylation 6 homolog (S. cerevisiae, alpha-1,3-glucosyltransferase)|asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3-glucosyltransferase)|asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog|asparagine-linked glycosylation protein 6 homolog|dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase|dolichyl-P-Glc:Man(9)GlcNAc(2)-PP-dolichol alpha- 1->3-glucosyltransferase|dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase|dolichyl-P-Glc:Man9GlcNAc2-PP-dolichylglucosyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALG6, check out the ALG6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALG6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ALG6 Antibody (A07747)
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No publications found for A07747
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-ALG6 Antibody
Question
Would anti-ALG6 antibody A07747 work on monkey IHC with fetal brain?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
Our lab technicians have not tested anti-ALG6 antibody A07747 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-ALG6 antibody A07747 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey fetal brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-13
Question
We are currently using anti-ALG6 antibody A07747 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The anti-ALG6 antibody (A07747) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-29
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-ALG6 antibody A07747. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-04
Question
I see that the anti-ALG6 antibody A07747 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-18