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Product Info Summary
| SKU: | M08374-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, ICC |
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Product info
Product Name
Anti-Human IgG3 IGHG3 Rabbit Monoclonal Antibody, Clone#RM119
SKU/Catalog Number
M08374-1
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgG3 IGHG3 Rabbit Monoclonal Antibody, Clone#RM119 (Catalog # M08374-1). Tested in ELISA, ICC, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgG3 IGHG3 Rabbit Monoclonal Antibody, Clone#RM119 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08374-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM119
Isotype
Rabbit IgG
Immunogen
Human IgG3
Cross-reactivity
Reacts to the heavy chain of human IgG3, RM119 does not cross-react to any other IgG subclasses (IgG1, IgG2, IgG4), and shows no cross-reactivity IgM, IgA, IgD, IgE. RM119 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Reactive Species
M08374-1 is reactive to IGHG3 in Human
Calculated molecular weight
41.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08374-1 is guaranteed for ELISA, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection)
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL.
Validation Images & Assay Conditions
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ELISA result showing specificity
Detection of human IgG3 in monkey serum. Sandwich ELISA using RM119 as the capture antibody, and biotinylated anti-human light chains ( or ) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.
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ELISA result showing specificity
Sandwich ELISA using RM119 as the capture antibody (100ng/well), and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin.
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ELISA result showing specificity
ELISA of human immunoglobulins shows RM119 only reacted to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM119 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody
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ELISA result showing specificity
A titer ELISA using RM119. The plate was coated with different amounts of human IgG3. A serial dilution of RM119 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA result showing specificity
ELISA showing RM119 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM119 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human lymphoid tissue section using anti-human IgG3 rabbit monoclonal antibody (Clone RM119).
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IHC result
Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG3 antibody RM119 at a 1:2000 dilution.
Specific Publications For Anti-Human IgG3 IGHG3 Rabbit Monoclonal Antibody, Clone#RM119 (M08374-1)
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1 Customer Q&As for Anti-Human IgG3 IGHG3 Rabbit Monoclonal Antibody, Clone#RM119
Question
We are currently using anti-Human IgG3 Rabbit Monoclonal antibody, Clone#RM119 M08374-1 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-08
Answer
The anti-Human IgG3 Rabbit Monoclonal antibody, Clone#RM119 (M08374-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-08
