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Product Info Summary
| SKU: | A02431 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-14-3-3 beta YWHAB Antibody
SKU/Catalog Number
A02431
Size
100μl
Form
Liquid
Description
Boster Bio Anti-14-3-3 beta YWHAB Antibody catalog # A02431. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-14-3-3 beta YWHAB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02431)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human 14-3-3 beta. AA range:41-90
Reactive Species
A02431 is reactive to YWHAB in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
28.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02431 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using 14-3-3 β Polyclonal Antibody
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Western Blot analysis of Jurkat cells using 14-3-3 β Polyclonal Antibody
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Western blot analysis of lysates from HepG2 and Jurkat cells, using 14-3-3 beta Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HepG2 cells using 14-3-3 β antibody.
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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200
Specific Publications For Anti-14-3-3 beta YWHAB Antibody (A02431)
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