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Product Info Summary
| SKU: | M11385-3 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA |
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Product info
Product Name
Anti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217
SKU/Catalog Number
M11385-3
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217 (Catalog # M11385-3). Tested in ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11385-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM217
Isotype
Rabbit IgG
Immunogen
Peptide corresponding to the Fc region of Human IgG4
Cross-reactivity
This antibody reacts to the Fc region of human IgG4. No cross-reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Reactive Species
M11385-3 is reactive to IGHG4 in Human
Calculated molecular weight
35.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11385-3 is guaranteed for ELISA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 50ng/well – 200ng/well (for Capture)
0.05ug/mL – 0.2ug/mL (for Detection).
Validation Images & Assay Conditions
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Specificity Test result
RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3
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ELISA result showing specificity
Sandwich ELISA using RM217 as the capture antibody, and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
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ELISA result showing specificity
Sandwich ELISA using RM217 as the capture antibody, and Biotinylated anti-human light chains (+) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
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ELISA result showing specificity
A titer ELISA using RM217. The plate was coated with different amounts of human IgG4. A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Specific Publications For Anti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217 (M11385-3)
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Customer Q&As
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1 Customer Q&As for Anti-Human IgG4 Fc IGHG4 Rabbit Monoclonal Antibody, Clone#RM217
Question
We are currently using anti-Human IgG4 Fc Rabbit Monoclonal antibody, Clone#RM217 M11385-3 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-05
Answer
The anti-Human IgG4 Fc Rabbit Monoclonal antibody, Clone#RM217 (M11385-3) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-05
