Human TNFRSF13B/TACI ELISA Kit PicoKine™

Human TNFRSF13B/TACI ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF13B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0986
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human TNFRSF13B/TACI ELISA Kit PicoKine™

See all TACI/TNFRSF13B/CVID products

SKU/Catalog Number

EK0986

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TNFRSF13B/TACI ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF13B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TNFRSF13B/TACI ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0986)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S2-T166

Sensitivity

<10 pg/ml

Assay Range

93.7 pg/ml - 6,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0986 is reactive to TNFRSF13B in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0986 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0986-CAP Anti-Human TNFRSF13B Pre-coated 96-well strip microplate 1
EK0986-ST Human TNFRSF13B Standard 2 vials, 10 ng/tube
EK0986-DA Human TNFRSF13B Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)093.7187.53757501300030006000
O.D.0.0360.1120.1600.3020.5140.9771.6862.343

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TNFRSF13B/TACI ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)18090725961628752578
Standard deviation13.6839134.9913.1248.12167.57
CV (%)7.6%4.3%5.2%8.1%5.5%6.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TACI/TNFRSF13B/CVID in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11801841901731816.173.4%
Sample 290778983181583543.915.2%
Sample 325963026263627632755168.036%
*number of samples for each test n=16.

Gene/Protein Information For TNFRSF13B (Source: Uniprot.Org, NCBI)

Uniprot ID

O14836

Gene ID

23495

Gene Name

TNFRSF13B

Full Name

Tumor necrosis factor receptor superfamily member 13B

Weight

31.816kDa

Alternative Names

CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B TNFRSF13B CD267, CVID, CVID2, IGAD2, RYZN, TACI, TNFRSF14B TNF receptor superfamily member 13B tumor necrosis factor receptor superfamily member 13B|transmembrane activator and CAML interactor|tumor necrosis factor receptor 13B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNFRSF13B, check out the TNFRSF13B Infographic

TNFRSF13B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNFRSF13B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0986 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Characteristics of CD4+CD25+Foxp3+?regulatory T cells in patients with multiple organ dysfunction syndrome

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6 Customer Q&As for Human TNFRSF13B/TACI ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated TACI antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TACI antigen?

Verified Customer

Verified customer

Asked: 2020-08-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-08-25

Question

Q: What is the optimal O.D. value for TACI ELISA kit? I performed your TACI ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TACI even though the O.D. values are not very high?

L. White

Verified customer

Asked: 2020-01-13

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TACI ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TACI that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-01-13

Question

Q: how do I analyze ELISA data? I have performed TACI level in plasma.

Verified Customer

Verified customer

Asked: 2019-09-15

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-09-15

Question

Q: can I use citrate plasma as samples in Human TACI Picokine® ELISA Kit (Catalog # EK0986)?

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TACI causing degradation of its protein structure. TACI may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TACI ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-05-22

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2017-01-15

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2017-01-15

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

K. Rodriguez

Verified customer

Asked: 2016-07-21

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-07-21

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