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Product Info Summary
| SKU: | EK0986 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human TNFRSF13B/TACI ELISA Kit PicoKine®
View all TACI/TNFRSF13B/CVID ELISA kits
SKU/Catalog Number
EK0986
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TNFRSF13B/TACI ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF13B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TNFRSF13B/TACI ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0986)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S2-T166
Sensitivity
<10 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0986 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0986 is reactive to TNFRSF13B in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0986 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TACI/TNFRSF13B/CVID
Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system. TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis. TACI is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand (CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0986-CAP | Anti-Human TNFRSF13B Pre-coated 96-well strip microplate | 1 |
| EK0986-ST | Human TNFRSF13B Standard | 2 vials, 10 ng/tube |
| EK0986-DA | Human TNFRSF13B Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 13000 | 3000 | 6000 |
| O.D. | 0.036 | 0.112 | 0.160 | 0.302 | 0.514 | 0.977 | 1.686 | 2.343 |
Data Example Images
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Human TNFRSF13B/TACI PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TACI/TNFRSF13B/CVID, Dilution ratio of 1:1, concentration in serum and plasma is around 300 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TNFRSF13B/TACI ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 180 | 907 | 2596 | 162 | 875 | 2578 |
| Standard deviation | 13.68 | 39 | 134.99 | 13.12 | 48.12 | 167.57 |
| CV (%) | 7.6% | 4.3% | 5.2% | 8.1% | 5.5% | 6.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TACI/TNFRSF13B/CVID in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 180 | 184 | 190 | 173 | 181 | 6.17 | 3.4% |
| Sample 2 | 907 | 789 | 831 | 815 | 835 | 43.91 | 5.2% |
| Sample 3 | 2596 | 3026 | 2636 | 2763 | 2755 | 168.03 | 6% |
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF13B (Source: Uniprot.Org, NCBI)
Gene Name
TNFRSF13B
Full Name
Tumor necrosis factor receptor superfamily member 13B
Weight
31.816kDa
Alternative Names
CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B TNFRSF13B CD267, CVID, CVID2, IGAD2, RYZN, TACI, TNFRSF14B TNF receptor superfamily member 13B tumor necrosis factor receptor superfamily member 13B|transmembrane activator and CAML interactor|tumor necrosis factor receptor 13B
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFRSF13B, check out the TNFRSF13B Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF13B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TNFRSF13B/TACI ELISA Kit PicoKine® (EK0986)
Hello CJ!
EK0986 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome
Prognostic Significance of Serum BAFF, APRIL, TACI and BCMA Levels in Chronic Lymphocytic Leukemia
Characteristics of CD4+CD25+Foxp3+?regulatory T cells in patients with multiple organ dysfunction syndrome
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Customer Q&As
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6 Customer Q&As for Human TNFRSF13B/TACI ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated TACI antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TACI antigen?
Verified Customer
Verified customer
Asked: 2020-08-25
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-08-25
Question
Q: What is the optimal O.D. value for TACI ELISA kit? I performed your TACI ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TACI even though the O.D. values are not very high?
L. White
Verified customer
Asked: 2020-01-13
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TACI ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TACI that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-01-13
Question
Q: how do I analyze ELISA data? I have performed TACI level in plasma.
Verified Customer
Verified customer
Asked: 2019-09-15
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-09-15
Question
Q: can I use citrate plasma as samples in Human TACI Picokine® ELISA Kit (Catalog # EK0986)?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TACI causing degradation of its protein structure. TACI may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TACI ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-05-22
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2017-01-15
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-01-15
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
K. Rodriguez
Verified customer
Asked: 2016-07-21
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-07-21
