Mouse TNFRSF13B/TACI ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TNFRSF13B/TACI. 96wells/kit, with removable strips.
Mouse TNFRSF13B/TACI ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Mouse TNFRSF13B/TACI ELISA Kit PicoKine™
See all TACI/TNFRSF13B/CVID primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse TNFRSF13B with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. TNFRSF13B tissue specificity:|
|Cite This Product||Mouse TNFRSF13B/TACI ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1144)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: A2-C128|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Tnfrsf13b (Source: Uniprot.org, NCBI)
|NCBI Gene Id||57916|
|Species Of This Entry||Mouse|
|Protein Name||Tumor necrosis factor receptor superfamily member 13B|
|Alternative Names||TACI/TNFRSF13B/CVID|CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Ubiquitination|
|Gene Location||11|11 B2, on Chromasome 11, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Tnfrsf13b (Source: Uniprot.org, NCBI)
|Protein Function||Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T- cell function and the regulation of humoral immunity (By similarity).|
|Research Areas||Apoptosis; Signal Transduction|
|Related Diseases||Immunologic Deficiency Syndromes; Common Variable Immunodeficiency; Autoimmune Reaction; Autoimmune Diseases; Autoimmunity; Malignant Neoplasms; Neoplasms; Infective Disorder; Immunoglobulin A Deficiency (disorder); Lupus Erythematosus, Systemic; Lymphoma; Multiple Myeloma; Malignant Paraganglionic Neoplasm; Primary Immune Deficiency Disorder; Inflammation; B-cell Lymphomas; Rheumatoid Arthritis; Arthritis; Agammaglobulinemia; Hyperplasia|
|Related Pathways||Cell Maturation; Pathogenesis; Cell Activation; Secretion; Cell Proliferation; Cell Differentiation; B Cell Activation; Immunoglobulin Production; Cell Development; B Cell Proliferation; Isotype Switching; B Cell Homeostasis; Cell Growth; Immune Response; B Cell Differentiation; Plasma Cell Differentiation; T Cell Activation; Cell Cycle; Humoral Immune Response; Localization|
|Background||Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system. TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis. TACI is a lymphocyte-specific member of the tumor necrosis factor(TNF) receptor superfamily. It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand(CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.|
|Scientific References||PMID: 10881172 by Yan M., et al. Identification of a receptor for BLyS demonstrates a crucial role in humoral immunity.|
PMID: 10880535 by Xia X.-Z., et al. TACI is a TRAF-interacting receptor for TALL-1, a tumor necrosis factor family member involved in B cell regulation.
Kit Components And Assay QC Data Details Of Mouse TNFRSF13B/TACI ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1144-CAP||96-well plate precoated with anti-Mouse TACI/TNFRSF13B/CVID antibody||1|
|EK1144-ST||lyophilized recombinant Mouse TACI/TNFRSF13B/CVID standard||10ng/tube|
|EK1144-DA||biotinylated anti-Mouse TACI/TNFRSF13B/CVID antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1144 Mouse TNFRSF13B/TACI ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse TNFRSF13B/TACI ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse TNFRSF13B/TACI ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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