Mouse TNFRSF13B/TACI ELISA Kit PicoKine™

Mouse TNFRSF13B/TACI ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf13b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1144
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse TNFRSF13B/TACI ELISA Kit PicoKine™

See all TACI/TNFRSF13B/CVID products

SKU/Catalog Number

EK1144

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TNFRSF13B/TACI ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf13b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse TNFRSF13B/TACI ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1144)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A2-C128

Sensitivity

<10 pg/ml

Assay Range

46.9 pg/ml - 3,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1144 is reactive to Tnfrsf13b in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1144 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1144-CAP Anti-Mouse Tnfrsf13b Pre-coated 96-well strip microplate 1
EK1144-ST Mouse Tnfrsf13b Standard 2 vials, 10 ng/tube
EK1144-DA Mouse Tnfrsf13b Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)046.99418837575015003000
O.D.0.0320.0970.1540.2350.4090.7221.3292.243

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TACI/TNFRSF13B/CVID, Dilution ratio of 1:1, concentration in serum is NO-10pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TNFRSF13B/TACI ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)883271327963111224
Standard deviation4.9222.8974.317.122.788.12
CV (%)7%%7%5.6%7.4%7.3%7.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TACI/TNFRSF13B/CVID in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 188918995902.682.9%
Sample 232738136936736120.345.6%
Sample 31327138114201248134464.514.7%
*number of samples for each test n=16.

Gene/Protein Information For Tnfrsf13b (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9ET35

Gene ID

57916

Gene Name

Tnfrsf13b

Full Name

Tumor necrosis factor receptor superfamily member 13B

Weight

26.947kDa

Alternative Names

CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B Tnfrsf13b|1200009E08Rik, Ta, Taci|tumor necrosis factor receptor superfamily, member 13b|tumor necrosis factor receptor superfamily member 13B|transmembrane activator and CAML interactor|transmembrane activator and calcium-modulating and cyclophilin ligand interactor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnfrsf13b, check out the Tnfrsf13b Infographic

Tnfrsf13b infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnfrsf13b: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Mouse TNFRSF13B/TACI ELISA Kit PicoKine™

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of TACI in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-11-05

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TACI ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-11-05

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

D. Kulkarni

Verified customer

Asked: 2020-03-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-03-22

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring TACI using the EK1144 Mouse TACI Picokine® ELISA Kit?

T. Bhasin

Verified customer

Asked: 2020-01-16

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2020-01-16

Question

Q: Can TACI ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-08-11

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-08-11

Question

Q: can I use citrate plasma as samples in Mouse TACI Picokine® ELISA Kit (Catalog # EK1144)?

S. Babu

Verified customer

Asked: 2018-12-31

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of TACI causing disruption of its protein structure. TACI may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TACI ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-12-31

Question

Q: how to analyze ELISA data? I have obtained TACI level in serum.

M. Rodriguez

Verified customer

Asked: 2018-05-17

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-05-17

Question

Q: if the enzyme conjugated TACI antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TACI antigen?

Verified Customer

Verified customer

Asked: 2016-11-16

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-11-16

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