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Product Info Summary
| SKU: | A02056-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-IFN- alphaR2 IFNAR2 Antibody
SKU/Catalog Number
A02056-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-IFN- alphaR2 IFNAR2 Antibody catalog # A02056-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IFN- alphaR2 IFNAR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02056-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the N-terminal region of human IFNAR2. AA range:41-90
Reactive Species
A02056-1 is reactive to IFNAR2 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
57.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02056-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of AD293 cells using IFN-αR2 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from AD293 cells, using IFNAR2 Antibody.
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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
Specific Publications For Anti-IFN- alphaR2 IFNAR2 Antibody (A02056-1)
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Customer Q&As
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4 Customer Q&As for Anti-IFN- alphaR2 IFNAR2 Antibody
Question
I see that the anti-IFN- alphaR2 antibody A02056-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-09-17
Question
Would A02056-1 anti-IFN- alphaR2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
You can see on the product datasheet, A02056-1 anti-IFN- alphaR2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-07
Question
I was wanting to use your anti-IFN- alphaR2 antibody for IHC for human monocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human monocyte identification?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
As indicated on the product datasheet, A02056-1 anti-IFN- alphaR2 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human monocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-04
Question
Does anti-IFN- alphaR2 antibody A02056-1 work on bovine WB with cervix carcinoma?
A. Johnson
Verified customer
Asked: 2014-03-31
Answer
Our lab technicians have not validated anti-IFN- alphaR2 antibody A02056-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-IFN- alphaR2 antibody A02056-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-03-31
