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- Table of Contents
IGF1 antibody (A00148) performs well in Flow Cytometry, IHC, ELISA (Cap) and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Flow Cytometry analysis of Hela cells using anti-IGF1 antibody (A00148).
Overlay histogram showing Hela cells stained with A00148 (Blue line).The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IGF1 Antibody (A00148, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Hela cells stained with A00148 (Blue line).The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IGF1 Antibody (A00148, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-IGF1 Antibody
IGF1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IHC, ELISA (Cap) |
Tested Applications
IGF1 antibody was positively detected by IHC in:
human pancreatic cancer tissue, human liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
IGF1 antibody was positively detected by FC in:
Hela cell
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA (Cap)
1-5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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Boster Guarantee:What is this?
IHC
IF
Human
Tested
Recommend
Bovine
Predicted
Predicted
Dog
Predicted
Predicted
Monkey
Predicted
Predicted
Rabbit
Predicted
Predicted
Sheep
Predicted
Predicted
Rat
Predicted
Predicted
Mouse
Predicted
Predicted
Product Information
Product Name
Anti-IGF1 Antibody
View all IGF1 Antibodies
View all IGF1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human IGF1 recombinant protein (Position: P50-A118).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated Molecular Weight
21.8 kDa
Background of IGF1
Insulin-like growth factor 1 (IGF-1) also known as somatomedin C or mechano growth factor is a protein that in humans is encoded by the IGF1 gene. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons.Justice et al. (1990) placed the mouse IGF1 gene on chromosome 10.
Cite This Product
Anti-IGF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00148)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Cow
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Rabbit
99%
High Homology
Sheep
99%
High Homology
Rat
96%
High Homology
Mouse
94%
High Homology
Zebrafish
81%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for IGF1 antibody
Flow Cytometry (FC) protocol for IGF1 antibody
Publications
Application:
Species:
Long-pulse gastric electrical stimulation protects interstitial cells of Cajal in diabetic rats via IGF-1 signaling pathway
Published: 2016-06-21
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Application: WB
Impact Factor: 2.79
Species: Rat
PMID: 27340351
IGF-1 Antibody Prolongs the Effective Duration Time of Botulinum Toxin in Decreasing Muscle Strength
Published: 2013-04-25
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 2.46
Species: Rat
PMID: 23698763
Changes in serum IGF‑1 level and tumor VEGF expression in mice with colorectal cancer under hyperglycemic conditions
Published: 2013-02-26
Journal: Molecular Medicine Reports
Impact Factor: 1.17
PMID: 23446884
Polysaccharides derived from Lycium barbarum suppress IGF-1-induced angiogenesis via PI3K/HIF-1α/VEGF signalling pathways in MCF-7 cells
Published: 2011-10-18
Journal: FOOD CHEMISTRY
Application: WB
Impact Factor: 3.46
Species: Human
The expressions of IGF-1, BMP-2 and TGF-β1 in cartilage of condylar hyperplasia
Published: 2010-12-13
Journal: JOURNAL OF ORAL REHABILITATION
Application: IHC
Impact Factor: 1.48
Species: Human
PMID: 20626571
Development of cetuximab-resistant human nasopharyngeal carcinoma cell lines and mechanisms of drug resistance
Published: 2010-04-30
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 2.24
Species: Human
PMID: 20630698
Improvement in erectile dysfunction after insulin-like growth factor-1 gene therapy in diabetic rats
Published: 2007-01-08
Journal: ASIAN JOURNAL OF ANDROLOGY
Application: WB
Impact Factor: 1.74
Species: Rat
PMID: 16855763
Expression of recombinant triangular bream (Megalobrama terminalis) pre-IGF-I in Escherichia coli
Published: 2006-07-14
Journal: FISH PHYSIOLOGY AND BIOCHEMISTRY
Application: WB
Impact Factor: 0.49
Species: Megalobrama terminalis
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Product Promise
We promise Anti-IGF1 Antibody performs excellently in relevant applications for Flow Cytometry, IHC, ELISA (Cap). If it doesn't perform as promised, we will refund the product. Learn more
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A00148
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026