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Product Info Summary
| SKU: | PA1374 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Insulin-like growth factor I IGF1 Antibody Picoband®
SKU/Catalog Number
PA1374
BA0939 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Insulin-like growth factor I IGF1 Antibody catalog # PA1374. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Insulin-like growth factor I IGF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1374)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human IGF-1, different from the mouse and rat sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1374 is reactive to IGF1 in Human, Mouse, Rat
Observed Molecular Weight
50 kDa
Calculated molecular weight
21.8 kDa
Background of IGF1
Insulin-like growth factor 1 (IGF-1) also known as somatomedin C or mechano growth factor is a protein that in humans is encoded by the IGF1 gene. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons.Justice et al. (1990) placed the mouse IGF1 gene on chromosome 10.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1374 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human
Positive Control
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
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Anti-IGF-1 antibody, PA1374, Western blotting
WB: Recombinant Human IGF-1 Protein 2.5ng
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Anti-IGF-1 antibody, PA1374, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Analysis of the target genes of miR-18a in pancreatic progenitor cells. Schematic of the base pairing of miR-18a and its predicted target sequences on the 3′UTR of CTGF, Nedd9, IGF1, and CDK19 ( A ). miR-18a or NC, pMIR-target 3′UTR-luciferase or pMIR-target 3′UTR mut-luciferase, and Renilla pRL-SV40 vectors were co-transfected into 3T3 cells. Forty-eight hours later, Luciferase activity was determined using a Dual-Luciferase Reporter Assay System Kit. All Firefly luciferase values were normalized to the co-transfected Renilla luciferase values ( B ). Pancreatic progenitor cells were transfected with miR-18a or NC. Seventy-two hours after transfection, western blots were performed to determine the expression of CTGF, Nedd9, CDK19, and IGF1. β-actin was used as an endogenous control ( C ). Pancreatic progenitor cells were transfected with miR-18a or NC. Forty-eight hours after transfection, real-time quantitative RT-PCR was performed to determine the expression of CTGF, Nedd9, CDK19 , and IGF1 mRNA ( D ). The data from the miRNA transfected groups were normalized to that of control group. * P < 0.05, ** P < 0.01. Uncropped images for the blots were shown in .
Index in PubMed under a CC BY license. PMID: 28332553
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Effect of miR-18a target gene knock-down on the proliferation of pancreatic progenitor cells. Pancreatic progenitor cells were transfected with siCTGF-1/2, siNedd9-1/2, siCDK19-1/2, and siIGF1-1/2 or sicontrol (NC) for 72 h, and the knockdown of these genes was confirmed by western blot assay ( A ). The proliferation of pancreatic progenitor cells that knocked down the target genes were evaluated by CCK8 assay ( B ) and Ki67 immunofluorescent staining ( C ). The Ki67-positive cells were divided by DAPI-positive cells. The data from the siRNA transfected groups were normalized to that of control group. * P < 0.05, ** P < 0.01. Uncropped images for the blots were shown in .
Index in PubMed under a CC BY license. PMID: 28332553
Specific Publications For Anti-Insulin-like growth factor I IGF1 Antibody Picoband® (PA1374)
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3 Customer Q&As for Anti-Insulin-like growth factor I IGF1 Antibody Picoband®
Question
We are currently using anti-IGF1 antibody PA1374 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The anti-IGF1 antibody (PA1374) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-23
Question
I did not see a band in my samples at 8 kDa. Instead, I saw a very well-defined clear band at about 12.5 kDa. The positive control had a signal at 10 kDa. Does the mouse IGF-1 run higher than the human IGF-1? I used a 15% acrylamide gel.
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
A; There might be problem that your pre-stained markers did not give the required precision on your gel. Note that the nominal molecular weight of mouse IGF-1 is different from that of human.
Boster Scientific Support
Answered: 2019-10-25
Question
How should I reconstitute my WB control (IGF-1 recombinant protein 100 ng/vial) for working with the mouse muscle tissue? I have tried a couple of different dilutions already. It appeared that much of the protein stayed lodged at the top of the gel and did not migrate (Evidence from the signal saturation at the top of the blot in the control lane).
Verified Customer
Verified customer
Asked: 2016-10-04
Answer
We suggest you use 50 µL water to reconstitute with the 100 ng recombinant protein. Then mix 10 µL of this solution with 10 µL of 2X sample buffer so that the protein concentration is 1 ng/µl (i.e., 1 µg/mL).
Boster Scientific Support
Answered: 2016-10-04
