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Product Info Summary
| SKU: | A01144-3 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IL-1 alpha/IL1A Antibody
SKU/Catalog Number
A01144-3
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-IL-1 alpha/IL1A Antibody catalog # A01144-3. Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-IL-1 alpha/IL1A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01144-3)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human IL-1 alpha/IL1A recombinant protein (Position: K3-N269).
Reactive Species
A01144-3 is reactive to IL1A in Human, Mouse
Calculated molecular weight
30.6 kDa
Background of IL1A
Interleukin-1α (IL-1 alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01144-3 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
Immunocytochemistry/Immunofluorescence, 1:50-400
ELISA, 1:100-1000
Validation Images & Assay Conditions
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Specific Publications For Anti-IL-1 alpha/IL1A Antibody (A01144-3)
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