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- Table of Contents
Il10 antibody (M00021-1) performs well in IHC-P, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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IHC analysis of IL-10 using anti-IL-10 antibody (M00021-1).
IL-10 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml rat anti-IL-10 Antibody (M00021-1) overnight at 4°C. Biotinylated goat anti-rat IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
IL-10 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml rat anti-IL-10 Antibody (M00021-1) overnight at 4°C. Biotinylated goat anti-rat IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Anti- IL-10 Monoclonal Antibody
Il10 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rat | IHC-P, WB |
Tested Applications
Il10 antibody was positively detected by IHC in:
mouse spleen tissue, mouse lymphaden tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5&mu:g/ml
Mouse
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Product Information
Product Name
Anti- IL-10 Monoclonal Antibody
View all IL10 Antibodies
View all IL10 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rat
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AFIE-9
Isotype
Rat IgG1, κ
Immunogen
Recombinant mouse IL-10
Cross-Reactivity
No cross reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Calculated Molecular Weight
20.6 kDa
Background of Il10
Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.
Cite This Product
Anti- IL-10 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00021-1)
Protocols
Immunohistochemistry (IHC) protocol for Il10 antibody
Publications
Application:
Species:
Olfactory mucosa tissue-derived mesenchymal stem cells lysate ameliorates LPS-induced acute liver injury in mice
Published: 2022-11-11
Journal: BMC Pulmonary Medicine
Application: IHC
Impact Factor: 3.32
Species: Mouse
PMID: 36369030
Novel Multifunctional Silver Nanocomposite Serves as a Resistance-Reversal Agent to Synergistically Combat Carbapenem-Resistant Acinetobacter baumannii
Published: 2021-06-23
Journal: ACS Applied Materials & Interfaces
Application: WB
Impact Factor: 9.23
Species: Mouse
PMID: 34161080
Bufei Yishen Granules Combined with Acupoint Sticking Therapy Suppress Inflammation in Chronic Obstructive Pulmonary Disease Rats: Via JNK/p38 Signaling Pathway
Published: 2017-10-30
Journal: Evidence-based Complementary and Alternative Medicine
Application: IHC
Impact Factor: 1.74
Species: Mouse
PMID: 29234369
Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines of Scutellaria barbata D. Don in Lewis-Bearing C57BL/6 Mice
Published: 2015-05-03
Journal: Evidence-based Complementary and Alternative Medicine
Application: WB
Impact Factor: 1.88
Species: Mouse
PMID: 26064167
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Product Promise
We promise Anti- IL-10 Monoclonal Antibody performs excellently in relevant applications for IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00021-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026