Mouse IL-10/Interleukin-10 ELISA Kit PicoKine™

Mouse IL-10/Interleukin-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il10 in cell culture supernatants and serum. Sensitivity: 1pg/ml. Cited in 101 publication(s).

Product Info Summary

SKU: EK0417
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Mouse IL-10/Interleukin-10 ELISA Kit PicoKine™

See all IL-10 products

SKU/Catalog Number

EK0417

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-10/Interleukin-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il10 in cell culture supernatants and serum. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse IL-10/Interleukin-10 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0417)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S19-S178

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0417 is reactive to Il10 in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0417 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0417-CAP Anti-Mouse Il10 Pre-coated 96-well strip microplate 1
EK0417-ST Mouse Il10 Standard 2 vials, 10 ng/tube
EK0417-DA Mouse Il10 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0510.0950.1650.2670.4500.7581.2781.840

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-10, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IL-10 in samples: 24809259 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IL-10/Interleukin-10 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3321946635203420
Standard deviation1.8814.0119.12.6616.6425.7
CV (%)5.7%6.4%4.1%7.6%8.2%6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-10 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 133383837362.065.7%
Sample 221919320219020111.295.6%
Sample 346644840542743622.835.2%
*number of samples for each test n=16.

Gene/Protein Information For Il10 (Source: Uniprot.Org, NCBI)

Uniprot ID

P18893

Gene ID

16153

Gene Name

Il10

Full Name

Interleukin-10

Weight

20.641kDa

Superfamily

IL-10 family

Alternative Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF Il10|CSIF, IL-, If2a, Il-10|interleukin 10|interleukin-10|cytokine synthesis inhibitory factor|interferon 2a

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il10, check out the Il10 Infographic

Il10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0417 has been cited in 101 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Unsaturated polyurethane films grafted with enantiomeric polylysine promotes macrophage polarization to a M2 phenotype through PI3K/Akt1/mTOR axis
Species: Mouse

Terazosin activates Pgk1 and Hsp90 to promote stress resistance
Species: Mouse

Li H,Sun H,Xu Y,Xing G,Wang X.Curcumin plays a protective role against septic acute kidney injury by regulating the TLR9 signaling pathway.Transl Androl Urol.2021 May;10(5):2103-2112.doi:10.21037/tau-21-385.PMID:34159091;PMCID:PMC8185681.
Species: Rat
EK0417 usage in article: APP:ELISA, SAMPLE: SERUM, DILUTION:NA

Lv M,Xue G,Cheng H,Meng P,Lian X,Hölscher C,Li D. The GLP-1/GIP dual-receptor agonist DA5-CH inhibits the NF-κB inflammatory pathway in the MPTP mouse model of Parkinson‘s disease more effectively than the GLP-1 single-receptor agonist NLY01. Brain Behav. 2021 Jun 14. doi: 10.1002/brb3.2231. Epub ahead of print. PMID: 34125470.
Species: Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Feng Z,Peng S,Wu Z,Jiao L,Xu S,Wu Y,Liu Z,Hu Y,Liu J,Wu Y,Wang D.Ramulus mori polysaccharide-loaded PLGA nanoparticles and their anti-inflammatory effects in vivo.Int J Biol Macromol.2021 Jun 1:S0141-8130(21)01184-3.doi:10.1016/j.ijbiomac.2021.05.200.Epub ahead of print.PMID:34087293.
Species: Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:COLON, DILUTION:NA

Li D,Weng Y,Wang G,Zhen G.Anti-Septic Potential of 7-α-Obacunyl Acetate Isolated from the Toona sinensis on Cecal Ligation/Puncture Mice via Suppression of JAK-STAT/NF-κB Signal Pathway.Infect Drug Resist.2021;14:1813-1821https://doi.org/10.2147/IDR.S302853
Species: Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Chen J,Wang H,Jia L,He J,Li Y,Liu H,Wu R,Qiu Y,Zhan Y,Yuan Z,Cao Y,Li W,Xu K,Yin P.Bufalin targets the SRC-3/MIF pathway in chemoresistant cells to regulate M2 macrophage polarization in colorectal cancer.Cancer Lett.2021 May 14:S0304-3835(21)00223-8.doi:10.1016/j.canlet.2021.05.008.Epub ahead of print.PMID:34000344.
Species: Human,Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Wang Y,Gao Y,Huang Y,Pan Y,Yu Y,Zhou Y,Wan SS,Yang YN.The potential protective effects of miR-497 on corneal neovascularization are mediated via macrophage through the IL-6/STAT3/VEGF signaling pathway.Int Immunopharmacol.2021 May 10;96:107745.doi:10.1016/j.intimp.2021. 107745. Epub ahead of print.PMID:33984719.
Species: Human,Mouse
EK0417 usage in article: APP: ELISA, SAMPLE:CORNEAL TISSUE, DILUTION:NA

Yilun Luo,Xiaowen Zheng,Peiqi Yuan,Xingyao Ye,Lie Ma,Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes,Bioactive Materials,Volume 6,Issue 11,2021,Pages 4065-4072,ISSN 2452-199X,https://doi.org/10.1016/j.bioactmat.2021.04.018.
Species: Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:BMDM, DILUTION:NA

Duan Y,Zheng H,Li Z,Yao Y,Ding J,Wang X,Nakkala JR,Zhang D,Wang Z,Zuo X,Zheng X,Ling J,Gao C.Unsaturated polyurethane films grafted with enantiomeric polylysine promotes macrophage polarization to a M2 phenotype through PI3K/Akt1/mTOR axis.Biomaterials.2020 Jul;246:120012.doi:10.1016/j.biomaterials.2020.120012.Epub 2020 Mar 31.PMID:32276198.
Species: Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:MACROPHAGES, DILUTION:NA

Luo YY,Yang ZQ,Lin XF,Zhao FL,Tu HT,Wang LJ,Wen MY,Xian SX.Knockdown of lncRNA PVT1 attenuated macrophage M1 polarization and relieved sepsis induced myocardial injury via miR-29a/HMGB1 axis.Cytokine.2021 Apr 9:155509.doi:10.1016/j.cyto.2021.155509.Epub ahead of print.PMID:33840587.
Species: Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:BMDMS, DILUTION:NA

Feng Sun,Haiwei Zhang,Tianwen Huang,Jianhui Shi,Tianli Wei,Yansong Wang,"miRNA-221 Regulates Spinal Cord Injury-Induced Inflammatory Response through Targeting TNF-α Expression",BioMed Research International,vol.2021,Article ID 6687963,6 pages,2021.https://doi.org/10.1155/2021/6687963
Species: Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:SPINAL CORD TISSUE, DILUTION:NA

Zhu Y,Liang H,Liu X,Wu J,Yang C,Wong TM, Kwan KYH,Cheung KMC,Wu S,Yeung KWK.Regulation of macrophage polarization through surface topography design to facilitate implant-to-bone osteointegration. Sci Adv.2021 Apr 2;7(14):eabf6654.doi:10. 1126/sciadv.abf6654.PMID:33811079.
Species: Mouse,Rat
EK0417 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

Li-Bo Jiang, Sheng-Long Ding, Wang Ding, Di-Han Su, Fang-Xue Zhang, Tai-Wei Zhang, Xiao-Fan Yin, Lan Xiao, Yu-Lin Li, Feng-Lai Yuan, Jian Dong, Injectable Sericin Based Nanocomposite Hydrogel for Multi-modal Imaging-guided Immunomodulatory Bone Regeneration,Chemical Engineering Journal,2021,129323,ISSN 1385-8947,https://doi.org/10.1016/j.cej.2021.129323.
Species: Mouse,Rat

He Y, Peng H, Zhang H, Liu Y, Sun H. Structural characteristics and immunopotentiation activity of two polysaccharides from the petal of Crocus sativus. Int J Biol Macromol. 2021 Mar 4:S0141-8130(21)00523-7. doi: 10.1016/j.ijbiomac.2021.03. 006. Epub ahead of print. PMID: 33676979.
Species: Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

Xi Y,Ma Y,Xie B,Di A,Xu S,Luo X,Wang C,Dai H,Yan G,Qi Z.Vitamin D3 combined with antibody agents suppresses alloreactive memory T-cell responses to induce heart allograft long-term survival.Transpl Immunol.2021 Feb 13:101374.doi:10.1016/j.trim.2021.101374
Species: Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Zhang Z,Song X,Zhang Z,Li H,Duan Y,Zhang H,Lu H,Luo C, Wang M.The molecular characterization and immune protection of adhesion protein 65 (AP65) of Trichomonas vaginalis.Microb Pathog.2021 Jan 20:104750.doi:10.1016/j.micpath.2021.104750.Epub ahead of prin
Species: Mouse,Rat
EK0417 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Chen X,He Y,Zhu Y,Du J,Sun H.linc-AAM Facilitates Gene Expression Contributing to Macrophage Activation and Adaptive Immune Responses.Cell Rep.2021 Jan 5;34(1):108584.doi:10.1016/j.celrep.2020.108584.PMID:33406422.
Species: Human,Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

Xin Li,Licong Yang,Meng Xu,Gaoxiang Qiao,ChangJiang Li,Lezhen Lin,Guodong Zheng,Smilax china L. polyphenols alleviates obesity and inflammation by modulating gut microbiota in high fat/high sucrose diet-fed C57BL/6J mice,Journal of Functional Foods,Volume
Species: Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Mu,D,Li,D,Li,J,et al. Long non‐coding RNA HULC protects against atherosclerosis via inhibition of PI3K/AKT signaling pathway. IUBMB Life.2020;1– 11.https://doi.org/10.1002/iub.2417
Species: Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:AORTIC VASCULAR TISSUE, DILUTION:NA

Gao Y,Zhou X,Zhou Y,Zhang W,Zhao L.Chrysene accelerates the proceeding of chronic obstructive pulmonary disease with the aggravation of inflammation and apoptosis in cigarette smoke exposed mice.Hum Exp Toxicol.2020 Dec 20:960327120979343.doi:10. 1177/096
Species: Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:LUNG TISSUE, DILUTION:NA

Zhu J, Yang LK, Chen WL, Lin W, Wang YH, Chen T. Activation of SK/KCa Channel Attenuates Spinal Cord Ischemia-Reperfusion Injury via Anti-oxidative Activity and Inhibition of Mitochondrial Dysfunction in Rabbits. Front Pharmacol. 2019 Apr 2;10:325. doi: 1
Species: Rabbit

Zheng B, Ying M, Xie J, Chen Y, Wang Y, Ding X, Hong J, Liao W, Yu Q. A Ganoderma atrum polysaccharide alleviated DSS-induced ulcerative colitis by protecting the apoptosis/autophagy-regulated physical barrier and the DC-related immune barrier. Food Funct
Species: Mouse
EK0417 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA

Sun H, Fei L, Zhu B, Shi M. Quick and improved immune responses to inactivated H9N2 avian influenza vaccine by purified active fraction of Albizia julibrissin saponins. BMC Vet Res. 2020 Nov 7;16(1):427. doi: 10.1186/s12917-020-02648-1. PMID: 33160337; PM
Species: Mouse,Chicken
EK0417 usage in article: APP:ELISA, SAMPLE:SPLENOCYTES, DILUTION:NA

MiR-137 attenuates spinal cord injury by modulating NEUROD4 through reducing inflammation and oxidative stress

Detoxication mechanisms of Radix Tripterygium wilfordii via compatibility with Herba Lysimachia christinae in S180-bearing mice by involving Nrf2

Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis

Toxoplasma gondii excretory/secretory antigens (TgESAs) suppress pro-inflammatory cytokine secretion by inhibiting TLR-induced NF-?B activation in LPS-stimulated murine macrophages

Effects of Recombinant Toxoplasma gondii Citrate Synthase I on the Cellular Functions of Murine Macrophages In vitro

Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice

Resveratrol protects against L-arginine-induced acute necrotizing pancreatitis in mice by enhancing SIRT1-mediated deacetylation of p53 and heat shock factor 1

Oroxylin A inhibits the generation of Tregs in non-small cell lung cancer

H9N2 Avian Influenza Virus Protein PB1 Enhances the Immune Responses of Bone Marrow-Derived Dendritic Cells by Down-Regulating miR375

Impact of bone marrow-derived mesenchymal stem cells on remodeling the lung injury induced by lipopolysaccharides in mice

In Vitro Anti-Inflammatory Effects of Three Fatty Acids from Royal Jelly

miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?

Keratin 8 limits TLR-triggered inflammatory responses through inhibiting TRAF6 polyubiquitination

Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration

MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3

4,4'-diaponeurosporene, a C30 carotenoid, effectively activates dendritic cells via CD36 and NF-?B signaling in a ROS independent manner

In vitro modeling of COPD inflammation and limitation of p38 inhibitor - SB203580

Inhibition of Acute Lung Injury by TNFR-Fc through Regulation of an Inflammation-Oxidative Stress Pathway

Genetically Engineered Virus Nanofibers as an Efficient Vaccine for Preventing Fungal Infection

Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica

Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells

Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model

Hybrid phage displaying SLAQVKYTSASSI induces protection against?Candida albicans?challenge in BALB/c mice

A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and?Staphylococcus aureus

Wang W, Liu M, Wang Y, Yang T, Li D, Ding F, Sun H, Bai G, Li Q. Evid Based Complement Alternat Med. 2018 Jan 28;2018:2305683. doi: 10.1155/2018/2305683. eCollection 2018. Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via No...

Lu C, Liu H, Jin X, Chen Y, Liang CL, Qiu F, Dai Z. Front Pharmacol. 2018 Feb 12;9:88. doi: 10.3389/fphar.2018.00088. eCollection 2018. Herbal Components of a Novel Formula PSORI-CM02 Interdependently Suppress Allograft Rejection and Induce CD8+CD...

Du, J., Chen, X., Wang, C., & Sun, H. (2017). Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha. Journal of Ethnopharmacology. Advance online publication. doi: 1...

Wang, Q., Dong, X., Wang, Y., Liu, M., Sun, A., Li, N.,..., & Li, X. (2017). Adolescent escitalopram prevents the effects of maternal separation on depression- and anxiety-like behaviours and regulates the levels of inflammatory cytokines in adult...

Zhang, M., Wu, X., Xu, Y., He, M., Yang, J., Li, J.,..., & Jia, J. (2017). The cystathionine β-synthase/hydrogen sulfide pathway contributes to microglia-mediated neuroinflammation following cerebral ischemia. Brain, Behavior, and Immunity. Advanc...

Wang Z, Xie J, Yang Y, Zhang F, Wang S, Wu T, Shen M, Xie M. Sci Rep. 2017 Jan 17;7:40402. doi: 10.1038/srep40402. Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated mac...

Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, Chaugai S, Zeldin Dc, Tang Jr, Wang Dw. Prostaglandins Other Lipid Mediat. 2015 Jan-Mar;116-117:64-75. Doi: 10.1016/J.Prostaglandins.2015.01.004. Epub 2015 Feb 14. Cyp Epoxygenase 2J2 Prevents Cardiac ...

Yang B, Zhou X, Lan C. Bmc Gastroenterol. 2015 Apr 1;15:43. Doi: 10.1186/S12876-015-0272-8. Changes Of Cytokine Levels In A Mouse Model Of Post-Infectious Irritable Bowel Syndrome.

Zhang Y, Leng Yf, Xue X, Zhang Y, Wang T, Kang Yq. World J Gastroenterol. 2011 Jan 14;17(2):254-9. Doi: 10.3748/Wjg.V17.I2.254. Effects Of Penehyclidine Hydrochloride In Small Intestinal Damage Caused By Limb Ischemia-Reperfusion.

Luo H,, Wang X,, Wang J,, Chen C,, Wang N,, Xu Z,, Chen S,, Zeng C,. Cardiovasc Diabetol. 2015 Jun 10;14:76. Doi: 10.1186/S12933-015-0239-7. Chronic Nf-??b Blockade Improves Renal Angiotensin Ii Type 1 Receptor Functions And Reduces Blood Pressure...

Wang T, Zhou Yt, Chen Xn, Zhu Ax, Wu Bh. World J Gastroenterol. 2014 Jul 28;20(28):9519-27. Doi: 10.3748/Wjg.V20.I28.9519. Remote Ischemic Postconditioning Protects Against Gastric Mucosal Lesions In Rats.

Zhang X, Liu F, Liu H, Cheng H, Wang W, Wen Q, Wang Y. Inflamm Res. 2011 Jun;60(6):569-75. Doi: 10.1007/S00011-010-0305-2. Epub 2011 Jan 19. Urinary Trypsin Inhibitor Attenuates Lipopolysaccharide-Induced Acute Lung Injury By Blocking The Activati...

Chen T, Liu W, Chao X, Zhang L, Qu Y, Huo J, Fei Z. Brain Res Bull. 2011 Feb 1;84(2):163-8. Doi: 10.1016/J.Brainresbull.2010.11.015. Epub 2010 Dec 4. Salvianolic Acid B Attenuates Brain Damage And Inflammation After Traumatic Brain Injury In Mice.

Li Xj, Liu Dp, Chen Hl, Pan Xh, Kong Qy, Pang Qf. Int Immunopharmacol. 2012 Feb;12(2):460-4. Doi: 10.1016/J.Intimp.2012.01.001. Epub 2012 Jan 15. Lactoferrin Protects Against Lipopolysaccharide-Induced Acute Lung Injury In Mice.

Xia J, Zhou Xl, Zhao Y, Zhu Yq, Jiang S, Ni Sz. Inflammation. 2013 Dec;36(6):1431-9. Doi: 10.1007/S10753-013-9683-2. Roles Of Lipoxin A4 In Preventing Paracetamol-Induced Acute Hepatic Injury In A Rabbit Model.

Tang Y, Liao Y, Kawaguchi-Sakita N, Raut V, Fakhrejahani E, Qian N, Toi M. J Hepatobiliary Pancreat Sci. 2011 Jul;18(4):551-8. Doi: 10.1007/S00534-010-0368-Z. Sinisan, A Traditional Chinese Medicine, Attenuates Experimental Chronic Pancreatitis In...

Chen W, Qi J, Feng F, Wang Md, Bao G, Wang T, Xiang M, Xie Wf. Neurochem Int. 2014 Mar;68:28-37. Doi: 10.1016/J.Neuint.2014.01.015. Epub 2014 Feb 12. Neuroprotective Effect Of Allicin Against Traumatic Brain Injury Via Akt/Endothelial Nitric Oxide...

Ye Xh, Wu Y, Guo Pp, Wang J, Yuan Sy, Shang Y, Yao Sl. Brain Res. 2010 Apr 6;1323:174-83. Doi: 10.1016/J.Brainres.2010.01.079. Epub 2010 Feb 4. Lipoxin A4 Analogue Protects Brain And Reduces Inflammation In A Rat Model Of Focal Cerebral Ischemia R...

Jin Y, Wan Y, Chen G, Chen L, Zhang Mq, Deng L, Zhang Jc, Xiong Xz, Xin Jb. Plos One. 2014 Oct 16;9(10):E111044. Doi: 10.1371/Journal.Pone.0111044. Ecollection 2014. Treg/Il-17 Ratio And Treg Differentiation In Patients With Copd.

Sereshki N, Gharagozloo M, Ostadi V, Ghahiri A, Roghaei Ma, Mehrabian F, Andalib Aa, Hassanzadeh A, Hosseini H, Rezaei A. Int J Fertil Steril. 2014 Apr;8(1):59-66. Epub 2014 Mar 9. Variations In T-Helper 17 And Regulatory T Cells During The Menstr...

Hualin C, Wenli X, Dapeng L, Xijing L, Xiuhua P, Qingfeng P. Inflammation. 2012 Jun;35(3):1087-93. Doi: 10.1007/S10753-011-9415-4. The Anti-Inflammatory Mechanism Of Heme Oxygenase-1 Induced By Hemin In Primary Rat Alveolar Macrophages.

Zhou X, Cao Y, Ao G, Hu L, Liu H, Wu J, Wang X, Jin M, Zheng S, Zhen X, Alkayed Nj, Jia J, Cheng J. Antioxid Redox Signal. 2014 Oct 20;21(12):1741-58. Doi: 10.1089/Ars.2013.5587. Epub 2014 Mar 13. Camkk??-Dependent Activation Of Amp-Activated Prot...

Sun Hx, Chen Lq, Zhang J, Chen Fy. J Ethnopharmacol. 2014 May 14;153(3):831-9. Doi: 10.1016/J.Jep.2014.03.052. Epub 2014 Mar 29. Anti-Tumor And Immunomodulatory Activity Of Peptide Fraction From The Larvae Of Musca Domestica.

Ruan Z, Liu S, Zhou Y, Mi S, Liu G, Wu X, Yao K, Assaad H, Deng Z, Hou Y, Wu G, Yin Y. Plos One. 2014 Jun 2;9(6):E97815. Doi: 10.1371/Journal.Pone.0097815. Ecollection 2014. Chlorogenic Acid Decreases Intestinal Permeability And Increases Expressi...

Meng A, Wang B, Zhang X, Qi N, Liu D, Wu J. Inflammation. 2015;38(3):1260-6. Doi: 10.1007/S10753-014-0093-X. Additive Suppression Of Lps-Induced Il-10 And Tnf-?? By Pre-Treatment Of Dexamethasone And Sb203580 In A Murine Alveolar Macrophage Cell L...

Chen W, Guo Y, Yang W, Zheng P, Zeng J, Tong W. Exp Brain Res. 2015 Oct;233(10):2823-31. Doi: 10.1007/S00221-015-4352-3. Epub 2015 Jun 13. Protective Effect Of Ginsenoside Rb1 On Integrity Of Blood-Brain Barrier Following Cerebral Ischemia.

Li Y, Li Sy, Li Js, Deng L, Tian Yg, Jiang Sl, Wang Y, Wang Yy. Biol Pharm Bull. 2012;35(10):1752-60. Epub 2012 Aug 3. A Rat Model For Stable Chronic Obstructive Pulmonary Disease Induced By Cigarette Smoke Inhalation And Repetitive Bacterial Infe...

Sun H, Zhang J, Chen F, Chen X, Zhou Z, Wang H. Carbohydr Polym. 2015 May 5;121:388-402. Doi: 10.1016/J.Carbpol.2014.12.023. Epub 2015 Jan 3. Activation Of Raw264.7 Macrophages By The Polysaccharide From The Roots Of Actinidia Eriantha And Its Mol...

Tang Jf, Lv Xh, Wang Xl, Sun J, Zhang Yb, Yang Ys, Gong Hb, Zhu Hl. Bioorg Med Chem. 2012 Jul 15;20(14):4226-36. Doi: 10.1016/J.Bmc.2012.05.055. Epub 2012 Jun 5. Design, Synthesis, Biological Evaluation And Molecular Modeling Of Novel 1,3,4-Oxadia...

Niu Z, Ma J, Chu H, Zhao Y, Feng W, Cheng Y. Neurosci Lett. 2012 Nov 7;529(2):112-7. Doi: 10.1016/J.Neulet.2012.09.034. Epub 2012 Sep 27. Melanocortin 4 Receptor Antagonists Attenuates Morphine Antinociceptive Tolerance, Astroglial Activation And ...

Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.

Zhang Gb, Tian Lq, Li Ym, Liao Yf, Li J, Bing Fh. Phytomedicine. 2013 Aug 15;20(11):964-8. Doi: 10.1016/J.Phymed.2013.04.009. Epub 2013 Jun 6. Protective Effect Of Homonojirimycin From Commelina Communis (Dayflower) On Influenza Virus Infection In...

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6 Customer Q&As for Mouse IL-10/Interleukin-10 ELISA Kit PicoKine™

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring IL-10 using the EK0417 Mouse IL-10 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-07-05

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2020-07-05

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-10-27

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-10-27

Question

Q: if the enzyme conjugated IL-10 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-10 antigen?

Verified Customer

Verified customer

Asked: 2019-10-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-10-25

Question

Dear Support: Just want to know what is the clone number of the Mouse IL-10 ELISA Kit I purchased, that the plates are coated with?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

The antibodies used in Mouse IL-10 ELISA Kit are goat polyclonal as capture antibody, and rat monoclonal [B-C40] for detection.

Boster Scientific Support

Answered: 2019-06-26

Question

Q: can you suggest the dilution ratio of serum samples for detection of IL-10 in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-07-25

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-10 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-07-25

Question

I purchased Mouse IL-10 ELISA Kit PicoKine, I just want to make sure if this kit works on mouse blood sample.

Verified Customer

Verified customer

Asked: 2015-02-16

Answer

The product Mouse IL-10 ELISA Kit PicoKine have been tested and validated in mouse plasma and serum, however we would not recommend to use it in whole blood.

Boster Scientific Support

Answered: 2015-02-16

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