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Product Info Summary
| SKU: | PA1354 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Interleukin-10 IL10 Antibody Picoband®
SKU/Catalog Number
PA1354
BA4317-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # PA1354. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-10 IL10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1354)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human IL10.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1354 is reactive to IL10 in Human
Observed Molecular Weight
60 kDa
Calculated molecular weight
20.5 kDa
Background of IL10
Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse Il 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1354 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-IL-10 antibody, PA1354, Western blotting
Lane 1: Recombinant Human IL-10 Protein 10ng
Lane 2: Recombinant Human IL-10 Protein 5ng
Lane 3: Recombinant Human IL-10 Protein 2.5ng
Specific Publications For Anti-Interleukin-10 IL10 Antibody Picoband® (PA1354)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Interleukin-10 IL10 Antibody Picoband®
Question
Would PA1354 anti-IL10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
It shows on the product datasheet, PA1354 anti-IL10 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-04
Question
We want to test anti-IL10 antibody PA1354 on human esophagus for research purposes, then I may be interested in using anti-IL10 antibody PA1354 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
The products we sell, including anti-IL10 antibody PA1354, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-06
Question
I see that the anti-IL10 antibody PA1354 works with WB, what is the protocol used to produce the result images on the product page?
O. Edwards
Verified customer
Asked: 2019-09-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-09-27
Question
We were well pleased with the WB result of your anti-IL10 antibody. However we have observed positive staining in esophagus secreted. using this antibody. Is that expected? Could you tell me where is IL10 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
From literature, esophagus does express IL10. Generally IL10 expresses in secreted. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510
Boster Scientific Support
Answered: 2019-06-25
Question
I was wanting to use your anti-IL10 antibody for WB for human esophagus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human esophagus identification?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
It shows on the product datasheet, PA1354 anti-IL10 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-24
Question
Is there a BSA free version of anti-IL10 antibody PA1354 available?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL10 antibody PA1354 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-29
Question
I have attached the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody PA1354. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-16
Question
We are currently using anti-IL10 antibody PA1354 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
The anti-IL10 antibody (PA1354) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-14
Question
Is this PA1354 anti-IL10 antibody reactive to the isotypes of IL10?
Verified Customer
Verified customer
Asked: 2018-12-27
Answer
The immunogen of PA1354 anti-IL10 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human IL10(46-60aa DAFSRVKTFFQMKDQ). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-27
Question
Is a blocking peptide available for product anti-IL10 antibody (PA1354)?
M. Kulkarni
Verified customer
Asked: 2018-08-31
Answer
We do provide the blocking peptide for product anti-IL10 antibody (PA1354). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-08-31
Question
Does anti-IL10 antibody PA1354 work for WB with esophagus?
A. Carter
Verified customer
Asked: 2018-06-19
Answer
According to the expression profile of esophagus, IL10 is highly expressed in esophagus. So, it is likely that anti-IL10 antibody PA1354 will work for WB with esophagus.
Boster Scientific Support
Answered: 2018-06-19
Question
My question regarding product PA1354, anti-IL10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Thomas
Verified customer
Asked: 2016-06-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1354 anti-IL10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-06-16
Question
Will anti-IL10 antibody PA1354 work on zebrafish WB with t-cell?
L. Edwards
Verified customer
Asked: 2013-10-29
Answer
Our lab technicians have not tested anti-IL10 antibody PA1354 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IL10 antibody PA1354 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-10-29
Question
We have seen staining in human esophagus. Do you have any suggestions? Is anti-IL10 antibody supposed to stain esophagus positively?
E. Johnson
Verified customer
Asked: 2013-09-19
Answer
Based on literature esophagus does express IL10. Based on Uniprot.org, IL10 is expressed in esophagus, t-cell, among other tissues. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510
Boster Scientific Support
Answered: 2013-09-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody PA1354. Let me know if you need anything else.
E. Parker
Verified customer
Asked: 2013-08-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-06
