Anti-Interleukin-10 IL10 Antibody

Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # PA1354. Tested in WB applications. This antibody reacts with Human. Cited in 17 publication(s).

Product Info Summary

SKU: PA1354
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Interleukin-10 IL10 Antibody

See all IL-10 products

SKU/Catalog Number

PA1354

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # PA1354. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interleukin-10 IL10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1354)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human IL10 (46-60aa DAFSRVKTFFQMKDQ).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1354 is reactive to IL10 in Human

Applications

PA1354 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For IL10 (Source: Uniprot.Org, NCBI)

Uniprot ID

P22301

Gene ID

3586

Gene Name

IL10

Full Name

Interleukin-10

Weight

20.517kDa

Superfamily

IL-10 family

Alternative Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF IL10 CSIF, GVHDS, IL-10A, TGIF, IL10 interleukin 10 interleukin-10|T-cell growth inhibitory factor|cytokine synthesis inhibitory factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL10, check out the IL10 Infographic

IL10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1354 has been cited in 17 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface

Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats

Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A

Re-polarizing Myeloid-derived Suppressor Cells (MDSCs) with Cationic Polymers for Cancer Immunotherapy

Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model

Wang Zh, Zeng Xy, Han Sp, Fan Gx, Wang Jy. Neurochem Res. 2012 Aug;37(8):1811-9. Doi: 10.1007/S11064-012-0795-0. Epub 2012 May 15. Interleukin-10 Of Red Nucleus Plays Anti-Allodynia Effect In Neuropathic Pain Rats With Spared Nerve Injury.

Shao Q, Li Y, Wang Q, Zhao J. Neurochem Res. 2015 Apr;40(4):733-9. Doi: 10.1007/S11064-015-1521-5. Epub 2015 Jan 24. Il-10 And Il-1?? Mediate Neuropathic-Pain Like Behavior In The Ventrolateral Orbital Cortex.

Wang R, Lu M, Zhang J, Chen S, Luo X, Qin Y, Chen H. J Exp Clin Cancer Res. 2011 May 20;30:62. Doi: 10.1186/1756-9966-30-62. Increased Il-10 Mrna Expression In Tumor-Associated Macrophage Correlated With Late Stage Of Lung Cancer.

Zhong B, Shen H, Sun X, Wang H, Zhang Y, Sun Z. Cancer Biother Radiopharm. 2012 May;27(4):252-8. Doi: 10.1089/Cbr.2011.1105. Additive Effects Of Ulinastatin And Docetaxel On Growth Of Breast Cancer Xenograft In Nude Mice And Expression Of Pge2, Il...

Zhan X, Jia L, Niu Y, Qi H, Chen X, Zhang Q, Zhang J, Wang Y, Dong L, Wang C. Biomaterials. 2014 Dec;35(38):10046-57. Doi: 10.1016/J.Biomaterials.2014.09.007. Epub 2014 Sep 22. Targeted Depletion Of Tumour-Associated Macrophages By An Alendronate-...

Jin Hb, Yang Yb, Song Yl, Zhang Yc, Li Yr. Mol Biol Rep. 2012 Dec;39(12):11005-9. Doi: 10.1007/S11033-012-2002-4. Epub 2012 Oct 8. Protective Roles Of Quercetin In Acute Myocardial Ischemia And Reperfusion Injury In Rats.

Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.

Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...

Huang Z, Zhang Z, Jiang Y, Zhang D, Chen J, Dong L, Zhang J. J Control Release. 2012 Mar 10;158(2):286-92. Doi: 10.1016/J.Jconrel.2011.11.013. Epub 2011 Nov 18. Targeted Delivery Of Oligonucleotides Into Tumor-Associated Macrophages For Cancer Imm...

Huang Z, Yang Y, Jiang Y, Shao J, Sun X, Chen J, Dong L, Zhang J. Biomaterials. 2013 Jan;34(3):746-55. Doi: 10.1016/J.Biomaterials.2012.09.062. Epub 2012 Oct 27. Anti-Tumor Immune Responses Of Tumor-Associated Macrophages Via Toll-Like Receptor 4 ...

Zhang Y, Leng Yf, Xue X, Zhang Y, Wang T, Kang Yq. World J Gastroenterol. 2011 Jan 14;17(2):254-9. Doi: 10.3748/Wjg.V17.I2.254. Effects Of Penehyclidine Hydrochloride In Small Intestinal Damage Caused By Limb Ischemia-Reperfusion.

Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...

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15 Customer Q&As for Anti-Interleukin-10 IL10 Antibody

Question

Would PA1354 anti-IL10 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

It shows on the product datasheet, PA1354 anti-IL10 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-04

Question

We want to test anti-IL10 antibody PA1354 on human esophagus for research purposes, then I may be interested in using anti-IL10 antibody PA1354 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

The products we sell, including anti-IL10 antibody PA1354, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-06

Question

I see that the anti-IL10 antibody PA1354 works with WB, what is the protocol used to produce the result images on the product page?

O. Edwards

Verified customer

Asked: 2019-09-27

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-27

Question

We were well pleased with the WB result of your anti-IL10 antibody. However we have observed positive staining in esophagus secreted. using this antibody. Is that expected? Could you tell me where is IL10 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

From literature, esophagus does express IL10. Generally IL10 expresses in secreted. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510

Boster Scientific Support

Answered: 2019-06-25

Question

I was wanting to use your anti-IL10 antibody for WB for human esophagus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human esophagus identification?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

It shows on the product datasheet, PA1354 anti-IL10 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human esophagus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-24

Question

Is there a BSA free version of anti-IL10 antibody PA1354 available?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL10 antibody PA1354 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-29

Question

I have attached the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody PA1354. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-16

Question

We are currently using anti-IL10 antibody PA1354 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

The anti-IL10 antibody (PA1354) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-14

Question

Is this PA1354 anti-IL10 antibody reactive to the isotypes of IL10?

Verified Customer

Verified customer

Asked: 2018-12-27

Answer

The immunogen of PA1354 anti-IL10 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human IL10(46-60aa DAFSRVKTFFQMKDQ). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-12-27

Question

Is a blocking peptide available for product anti-IL10 antibody (PA1354)?

M. Kulkarni

Verified customer

Asked: 2018-08-31

Answer

We do provide the blocking peptide for product anti-IL10 antibody (PA1354). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-08-31

Question

Does anti-IL10 antibody PA1354 work for WB with esophagus?

A. Carter

Verified customer

Asked: 2018-06-19

Answer

According to the expression profile of esophagus, IL10 is highly expressed in esophagus. So, it is likely that anti-IL10 antibody PA1354 will work for WB with esophagus.

Boster Scientific Support

Answered: 2018-06-19

Question

My question regarding product PA1354, anti-IL10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

N. Thomas

Verified customer

Asked: 2016-06-16

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1354 anti-IL10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-06-16

Question

Will anti-IL10 antibody PA1354 work on zebrafish WB with t-cell?

L. Edwards

Verified customer

Asked: 2013-10-29

Answer

Our lab technicians have not tested anti-IL10 antibody PA1354 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IL10 antibody PA1354 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish t-cell in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-10-29

Question

We have seen staining in human esophagus. Do you have any suggestions? Is anti-IL10 antibody supposed to stain esophagus positively?

E. Johnson

Verified customer

Asked: 2013-09-19

Answer

Based on literature esophagus does express IL10. Based on Uniprot.org, IL10 is expressed in esophagus, t-cell, among other tissues. Regarding which tissues have IL10 expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 1847510

Boster Scientific Support

Answered: 2013-09-19

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for esophagus using anti-IL10 antibody PA1354. Let me know if you need anything else.

E. Parker

Verified customer

Asked: 2013-08-06

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-08-06

Size

Total: $240

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PA1354
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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