IL10 antibody (PA1354) performs well in WB and shows reactivity with Human.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 2 Publications
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pa1354 1 WB anti il 10 antibody

Designing a Western blot for IL10? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the IL10 Western Blot Guide

Anti-Interleukin-10 IL10 Antibody Picoband®

IL10 antibody

Reactive SpeciesHostApplication
HumanRabbitWB

SKU: PA1354

Size: 100 μg

Bundle to save: I do not want free secondary ab
Price:
$370/100 μg

2 in stock. Ships Next Business Day.

Quantity: 1

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Guarantee / Product Promise

Tested Applications

IL10 antibody was positively detected by WB in:

recombinant human IL-10 protein

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Recommend Dilution

Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Product Information

Product Name
Anti-Interleukin-10 IL10 Antibody Picoband®
View all IL10 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human IL10.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated Molecular Weight

20.5 kDa

Background of IL10
Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse Il 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.
Cite This Product
Anti-Interleukin-10 IL10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1354)

Protocols

Western Blot protocol for IL10 antibody

Publications

Application:
Species:
Therapeutic potential of Banxia-Houpu Decoction in alleviating inflammation and myocardial injury in chronic intermittent hypoxia models
Published: 2025-07-12
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB,IHC
Impact Factor: 4.70
Species: Mouse,Rat
PMID: 40652588
Enhancing Fatty Acid Catabolism of Macrophages Within Aberrant Breast Cancer Tumor Microenvironment Can Re-establish Antitumor Function
Published: 2021-04-15
Journal: Frontiers in Cell and Developmental Biology
Application: WB
Impact Factor: 6.68
Species: Human
PMID: 33937269

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Product Promise

We promise Anti-Interleukin-10 IL10 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more

In stock
Order Product
PA1354
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 26, 2026