Anti-IL10 Antibody

Effects of Pseudoephedrine + emodin on the expression of TNF-α, IL-6, IL-1β, iNOS, IL-10, Arg-1 in LPS‐induced ALI rats. The contents of TNF-α ( A ), IL-6 ( B ), IL-1β ( C ), iNOS ( D ), IL-10 ( E ), Arg-1 ( F ) in the serum and BALF were determined using ELISA. Data were expressed as mean ± S.D. (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. control group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. LPS alone group. + p < 0.05, ++ p < 0.01, +++ p < 0.001 vs. combined treatment group (5 + 20 mg/kg)
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Effects of VIP on levels of inflammatory and anti-inflammatory cytokines in LPS + IFN-γ or IL-4 + IL-13 induced AMs cells. Macrophages were pre-treated with VIP(10 –6 ,10 –7 , 10 –8 mol/L) for 24 h, followed by LPS (100 ng/mL) + IFN-γ(20 ng/mL) or IL-4 (40 ng/mL) + IL-13(20 ng/mL) stimulation for 12 h. Cells were collected and the contents of TNF-α ( A ), IL-6 ( B ), IL-10 ( C ), Arg-1 ( D ) were determined using ELISA. (E-J) TNF-α, IL-6, iNOS, IL-10, Arg-1, Ym-1 mRNA expression was determined using Real-time PCR analysis. Data were expressed as mean ± S.D. (n = 3). # p < 0.05, ## p < 0.01, ### p < 0.001 vs. control group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. LPS alone group. +p < 0.05, + + p < 0.01, +++ p < 0.001 vs. IL-4 alone group
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VIP inhibited (M1) macrophage and activated (M2) macrophage in LPS + IFN-γ‐induced or IL-4 + IL-13-induced AMs. Effects of Pseudoephedrine + emodin on the expression of VIP in LPS‐induced ALI rats. A – D , F , H , I (M1) macrophage Marker CD86, IL-6 and (M2) macrophage Marker CD206, IL-10 expression was determined using immunofluorescence. E , G CD86, CD206 mRNA expression was determined using Real-time PCR analysis. J The contents of VIP in the serum of ALI rats were determined using ELISA. Data are expressed as mean ± S.D. (n = 3). ## p < 0.01, ### p < 0.001 vs. control group. *p < 0.05, **p < 0.01 vs. LPS alone group. +p < 0.05, ++ p < 0.01, +++ p < 0.001 vs. IL-4 alone group
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Pseudoephedrine + emodin Inhibited MAPK in LPS-induced acute lung injury in rats. A – D Western blot analysis was performed to detect p-P38, p-ERK1/2 and p-JNK1/2 protein expression. E – I TNF-α, IL-6, IL-1β, IL-10, Arg-1 mRNA expression was determined using Real-time PCR analysis. All data are expressed as mean ± S.D. (n = 3). ## p < 0.01, ### p < 0.001 vs. control group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. LPS alone group. + p < 0.05, ++ p < 0.01, +++ p < 0.001 vs. Combined treatment group (5 + 20 mg/kg)
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Effects of polysaccharide extract from XJEK on TNF-α, IL-1β and IL-10 of HUVECs induced by Ang II. ( a ) TNF-α level in supernatants of HUVECs; ( b ) IL-1β level in supernatants of HUVECs; ( c ) IL-10 level in supernatants of HUVECs. 1, blank control group; 2, Ang II (10 − 5 mol/L) group; 3, Ang II (10 − 5 mol/L) + AqE (0.15 mg/ml) group; 4, Ang II (10 − 5 mol/L) + AqE (0.3 mg/ml) group; 5, Ang II (10 − 5 mol/L) + AqE (0.6 mg/ml) group; 6, Ang II (10 − 5 mol/L) + AqE (1.2 mg/ml) group; 7, Ang II (10 − 5 mol/L) + XJEK (1.6 mg/ml) group. Data are expressed as mean ± SD, n = 6. ** P < 0.01 vs control group; ## P < 0.01 vs Ang II group
Index in PubMed under a CC BY license. PMID: 31196042

Effects of polysaccharide extract from XJEK on TNF-α, IL-1β and IL-10 in L -NAME-induced hypertensive mice. ( a ) TNF-α expression level in plasma. ( b ) IL-1β expression level in plasma. ( c ) IL-10 expression level in plasma. ( d ) IL-1β expression level in cardiac tissues. ( e ) TNF-α expression level in cardiac tissues. ( f ) IL-10 expression level in cardiac tissues. ( g ) Representative image of IL-1β immunocytochemistry. ( h ) Representative image of TNF-α immunocytochemistry.( i ) Representative image of IL-10 immunocytochemistry.1,negative group; 2,control group; 3, model group; 4, L -NAME+AqE group; 5, L -NAME+XJEK group; 6, L -NAME+fosinopril group. Data are presented as the mean ± SD ( n = 10). ** P < 0.01 vs. control group; # P < 0.05, ## P < 0.01 vs, model group
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SADS inhibited Raw264.7 and fibroblast-like synoviocyte inflammation in vitro. (A) The effect of SADS on the viability of Raw264.7. (B–D) RT-PCR analysis of TNF-α, IL-6 and IL-10 in Raw264.7 treated with SADS.(E) The effect of SADS on the viability of fibroblast-like synoviocytes. (F–H) RT-PCR analysis of TNF-α, IL-6 and IL-10 in fibroblast-like synoviocytes treated with SADS.(I) The phenotype of Raw264.7 was analyzed by flow cytometry.(J and K) Statistics of the proportion of M2 and M1 macrophages. (L and M) TNF-α protein expression level detection. ##P < 0.01 versus Ctrl; ∗∗P < 0.01versus LPS. n = 6.
Index in PubMed under a CC BY license. PMID: 40688514

Pathological evaluation of IL-1RA−/− mice synovial tissue. HE staining showed synovial hyperplasia (A) and inflammation (B). (C) IHC staining of TNF-α in synovial tissue. (D) IHC staining of IL-10 in synovial tissue.(E and F) Levels of TNF-α and IL-10 in the blood of IL1RA−/ -deficient mice. ##P < 0.01 versus Ctrl; ∗∗P < 0.01versus Model. n = 6 mice for each group. Control is wild-type mice, and the model is IL1RA−/− mice.
Index in PubMed under a CC BY license. PMID: 40688514

IHC analysis of IL10 using anti-IL10 antibody (A00021-3).
IL10 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IL10 Antibody (A00021-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IHC analysis of IL10 using anti-IL10 antibody (A00021-3).
IL10 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IL10 Antibody (A00021-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.