Anti-IL10 Antibody Picoband™

IL-10 antibody

Boster Bio Anti-IL10 Antibody Picoband™ catalog # A00021-3. Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Mouse, Rat. Cited in 7 publication(s).

Product Info Summary

SKU: A00021-3
Size: 100μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC

Product Name

Anti-IL10 Antibody Picoband™

View all IL-10 Antibodies

SKU/Catalog Number

A00021-3

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IL10 Antibody Picoband™ catalog # A00021-3. Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00021-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived rat IL10 recombinant protein (Position: K52-A170).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00021-3 is reactive to Il10 in Mouse, Rat

Applications

A00021-3 is guaranteed for ELISA, Flow Cytometry, IHC Boster Guarantee

Background of IL-10

Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Rat
Direct ELISA, 0.1-0.5μg/ml, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Il10 (Source: Uniprot.Org, NCBI)

Uniprot ID

P29456

Gene ID

25325

Gene Name

Il10

Full Name

Interleukin-10

Weight

20.426kDa

Superfamily

IL-10 family

Alternative Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF Il10|IL10X, If2a|interleukin 10|interleukin-10|CSIF|IL-10|cytokine synthesis inhibitory factor|interferon 2a

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il10, check out the Il10 Infographic

Il10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00021-3 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Regional tissue immune responses after sciatic nerve injury in rats

Human interleukin-10 gene inhibits acute rejection by triggering apoptosis in allograft vascular transplantation

Myocardial ischemic post-conditioning protects the lung against myocardial ischemia/reperfusion-induced damage by activating GSK-3β

Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1α expression

Inhibition of lipopolysaccharide‐mediated rat alveolar macrophage activation in vitro by antiflammin‐1

Targeted depletion of tumour-associated macrophages by an alendronate–glucomannan conjugate for cancer immunotherapy
Species: Mouse

Niu Z, Ma J, Chu H, Zhao Y, Feng W, Cheng Y. Neurosci Lett. 2012 Nov 7;529(2):112-7. Doi: 10.1016/J.Neulet.2012.09.034. Epub 2012 Sep 27. Melanocortin 4 Receptor Antagonists Attenuates Morphine Antinociceptive Tolerance, Astroglial Activation And ...

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Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A00021-3

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A00021-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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