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Il10 antibody (A00021-3) performs well in ELISA, IHC and shows reactivity with Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 26 Publications

a00021 3 il10 primary antibodies ihc testing 1_1

IHC analysis of IL10 using anti-IL10 antibody (A00021-3).
IL10 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IL10 Antibody (A00021-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Designing a Western blot for IL10? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the IL10 Western Blot Guide

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Anti-IL10 Antibody

Il10 antibody

26 Publications

Reactive Species	Host	Application
Mouse, Rat	Rabbit	ELISA, IHC

Tested Applications

Il10 antibody was positively detected by IHC in:

mouse spleen tissue, rat spleen tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Mouse

Tested

Recommend

Rat

Tested

Recommend

Product Information

Product Name

Anti-IL10 Antibody
View all IL10 Antibodies

Form

Lyophilized

Uniprot

P29456

Gene ID

25325

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived rat IL10 recombinant protein (Position: K52-A170).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Calculated Molecular Weight

20.4 kDa

Background of Il10

Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Cite This Product

Anti-IL10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00021-3)

Protocols

Immunohistochemistry (IHC) protocol for Il10 antibody

Review Protocol

Publications

Artemin Serves as a Novel Predictive Biomarker and Exerts a Protective Effect Against Preterm Birth

Published: 2026-07-09

Journal: Journal of Inflammation Research

Application: IHC

Impact Factor: 4.60

Species: Human,Mouse

PMID: 42454152

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Free Secondary Antibody

Anti-IL10 Antibody

Il10 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Artemin Serves as a Novel Predictive Biomarker and Exerts a Protective Effect Against Preterm Birth

Glucose/ROS-responsive and redox-gated adaptive hydrogel dressing for accelerating diabetic wound repair via synergistic cGAS/STING pathway inhibition and oxidative stress alleviation

Targeting Talin1 to reprogram dendritic cell activation landscapes: A mechanistically grounded cell therapy for multiple sclerosis model

Aqueous extract of Saposhnikovia divaricata root alleviates rheumatoid arthritis by acting on TNF-α and RAGE signaling pathways

Application of sodium alginate and polyethylene glycol bilayer multifunctional hydrogel microneedles in infectious and diabetic wounds

Facile Nanocomposite Hydrogel Scaffold with Sustained Drug Release and Osteo-Immunomodulatory Effects to Enhance Bone Regeneration

Multifunctional Drug- and AuNRs-Loaded ROS-Responsive Selenium-Containing Polyurethane Nanofibers for Smart Wound Healing

Reversing inflammatory microenvironment by a single intra-articular injection of multi-stimulus responsive lipogel to relieve rheumatoid arthritis and promote joint repair

A Synbiotic Ameliorates Con A-Induced Autoimmune Hepatitis in Mice through Modulation of Gut Microbiota and Immune Imbalance

Dietary genistein increases microbiota-derived short chain fatty acid levels, modulates homeostasis of the aging gut, and extends healthspan and lifespan

Efficient starvation therapy with three-pathway blocking in combination with PTT/CDT for TME reversal and tumor apoptosis

Combination of pseudoephedrine and emodin ameliorates LPS-induced acute lung injury by regulating macrophage M1/M2 polarization through the VIP/cAMP/PKA pathway

Epigenetic changes in inflammatory genes and the protective effect of cooked rhubarb on pancreatic tissue of rats with chronic alcohol exposure

Biomimetic hydrogel for rapid and scar-free healing of skin wounds inspired by the healing process of oral mucosa

The protective effects of polysaccharide extract from Xin-Ji-Er-Kang formula on Ang II-induced HUVECs injury, L-NAME-induced hypertension and cardiovascular remodeling in mice

Targeted Delivery of Zoledronate to Tumor-Associated Macrophages for Cancer Immunotherapy

Targeted Delivery of miRNA 155 to Tumor Associated Macrophages for Tumor Immunotherapy

Myocardial ischemic post-conditioning protects the lung against myocardial ischemia/reperfusion-induced damage by activating GSK-3β1

Regional tissue immune responses after sciatic nerve injury in rats

IL-10 and IL-1β Mediate Neuropathic-Pain Like Behavior in the Ventrolateral Orbital Cortex

Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1α expression

Human interleukin-10 gene inhibits acute rejection by triggering apoptosis in allograft vascular transplantation

Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model

Inhibition of lipopolysaccharide-mediated rat alveolar macrophage activation in vitro by antiflammin-1

Protective roles of quercetin in acute myocardial ischemia and reperfusion injury in rats

Melanocortin 4 receptor antagonists attenuates morphine antinociceptive tolerance, astroglial activation and cytokines expression in the spinal cord of rat

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