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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human ICAM-3 / CD50 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human ICAM-3 / CD50 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ICAM3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ICAM-3 / CD50 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0940)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Q30-H485; homodimer
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0940 is reactive to ICAM3 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0940 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ICAM-3/CD50
Intercellular adhesion molecule 3 (ICAM3) also known as CD50 (Cluster of Differentiation 50), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM3 has been shown to interact with EZR and Moesin.
|EK0940-CAP||Anti-Human ICAM3 Pre-coated 96-well strip microplate||1|
|EK0940-ST||Human ICAM3 Standard||2 vials, 10 ng/tube|
|EK0940-DA||Human ICAM3 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human ICAM-3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ICAM-3 / CD50 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of ICAM-3/CD50 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For ICAM3 (Source: Uniprot.Org, NCBI)
Intercellular adhesion molecule 3
CD50 antigen; CD50; CDW50CDw50; ICAM3; ICAM-3; ICAM-Rintercellular adhesion molecule-3; intercellular adhesion molecule 3 ICAM3 CD50, CDW50, ICAM-R intercellular adhesion molecule 3 intercellular adhesion molecule 3|ICAM-3*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ICAM3, check out the ICAM3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ICAM3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0940 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Huang Kf, Huang Xp, Xiao Gq, Yang Hy, Lin Js, Diao Y. Biomed Pharmacother. 2014 May;68(4):455-61. Doi: 10.1016/J.Biopha.2014.03.005. Epub 2014 Mar 18. Kallistatin, A Novel Anti-Angiogenesis Agent, Inhibits Angiogenesis Via Inhibition Of The Nf-??b...
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