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Product Info Summary
| SKU: | A01583-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB, ELISA (Cap) |
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Product info
Product Name
Anti-GDF15 Antibody Picoband®
SKU/Catalog Number
A01583-1
Size
100 μg/vial
Form
Lyophilized
Description
GDF15 is a TGF-β superfamily cytokine implicated in regulation of inflammatory and apoptotic pathways in injured tissues and during disease processes (stress-response biology; mechanistically linked to metabolic adaptation during systemic inflammation). Assay context: antibody is specified for Western blotting and capture ELISA in Human; often contextualized with other TGF-β superfamily signaling ligands such as BMP-2 (shared superfamily, distinct pathways) and may be profiled with vascular factors like PROK1 in injury/repair paradigms (putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GDF15 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01583-1)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GDF15 recombinant protein (Position: A195-I308). Human GDF15 shares 68.1% and 69.2% amino acid (aa) sequence identity with mouse and rat GDF15, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01583-1 is reactive to GDF15 in Human
Observed Molecular Weight
34 kDa
Calculated molecular weight
34.1 kDa
Background of GDF15
Growth/differentiation factor 15 (GDF15) is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB. GDF15 mRNA is most abundant in the liver, with lower levels seen in some other tissues. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01583-1 is guaranteed for WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
ELISA(Cap), 1-5 μg/ml, Human
Positive Control
WB: 22RV whole Cell, human placenta tissue
Validation Images & Assay Conditions
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Western blot analysis of GDF15 using anti-GDF15 antibody (A01583-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: 22RV whole Cell lysates,
Lane 2: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GDF15 antigen affinity purified polyclonal antibody (Catalog # A01583-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GDF15 at approximately 34KD. The expected band size for GDF15 is at 34KD.
Click image to see more details
Sandwich ELISA - Recombinant human GDF-15 protein standard curve.
Use in combination with reagents from Human GDF-15 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0767).
Specific Publications For Anti-GDF15 Antibody Picoband® (A01583-1)
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Customer Q&As
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15 Customer Q&As for Anti-GDF15 Antibody Picoband®
Question
We have seen staining in human fibrosarcoma. What should we do? Is anti-GDF15 antibody supposed to stain fibrosarcoma positively?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
According to literature fibrosarcoma does express GDF15. According to Uniprot.org, GDF15 is expressed in chorionic villus, placenta, fibrosarcoma, muscle, among other tissues. Regarding which tissues have GDF15 expression, here are a few articles citing expression in various tissues:
Fibrosarcoma, Pubmed ID: 9348093
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 9375789, 9593718, 14702039
Boster Scientific Support
Answered: 2020-04-28
Question
Is this A01583-1 anti-GDF15 antibody reactive to the isotypes of GDF15?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The immunogen of A01583-1 anti-GDF15 antibody is E.coli-derived human GDF15 recombinant protein (Position: A195-I308). Human GDF15 shares 68.1% and 69.2% amino acid (aa) sequence identity with mouse and rat GDF15, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-03
Question
Do you have a BSA free version of anti-GDF15 antibody A01583-1 available?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GDF15 antibody A01583-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-20
Question
Here is the WB image, lot number and protocol we used for placenta using anti-GDF15 antibody A01583-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-19
Question
Will A01583-1 anti-GDF15 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
As indicated on the product datasheet, A01583-1 anti-GDF15 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-24
Question
I see that the anti-GDF15 antibody A01583-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-06
Question
My team were happy with the WB result of your anti-GDF15 antibody. However we have been able to see positive staining in chorionic villus secreted using this antibody. Is that expected? Could you tell me where is GDF15 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
From literature, chorionic villus does express GDF15. Generally GDF15 expresses in secreted. Regarding which tissues have GDF15 expression, here are a few articles citing expression in various tissues:
Fibrosarcoma, Pubmed ID: 9348093
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 9375789, 9593718, 14702039
Boster Scientific Support
Answered: 2019-10-21
Question
Is a blocking peptide available for product anti-GDF15 antibody (A01583-1)?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
We do provide the blocking peptide for product anti-GDF15 antibody (A01583-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-20
Question
I was wanting to use your anti-GDF15 antibody for WB for human placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
You can see on the product datasheet, A01583-1 anti-GDF15 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-14
Question
Can you help my question with product A01583-1, anti-GDF15 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01583-1 anti-GDF15 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-03
Question
My question regards using your anti-GDF15 antibody for positive regulation of mapk cascade studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-08-14
Answer
We appreciate your inquiry. This A01583-1 anti-GDF15 antibody is validated on 22rv whole cell lysates, human placenta tissue. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-08-14
Question
We want to test anti-GDF15 antibody A01583-1 on human placenta for research purposes, then I may be interested in using anti-GDF15 antibody A01583-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Gonzalez
Verified customer
Asked: 2017-09-13
Answer
The products we sell, including anti-GDF15 antibody A01583-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-13
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-GDF15 antibody A01583-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-31
Question
Would anti-GDF15 antibody A01583-1 work for WB with placenta?
J. Li
Verified customer
Asked: 2014-06-10
Answer
According to the expression profile of placenta, GDF15 is highly expressed in placenta. So, it is likely that anti-GDF15 antibody A01583-1 will work for WB with placenta.
Boster Scientific Support
Answered: 2014-06-10
Question
We are currently using anti-GDF15 antibody A01583-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
E. Zhao
Verified customer
Asked: 2013-01-01
Answer
The anti-GDF15 antibody (A01583-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-01-01
