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Product Info Summary
| SKU: | M00368-4 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-iNOS/NOS2 Antibody (Monoclonal, 31N04)
SKU/Catalog Number
M00368-4
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-iNOS/NOS2 Antibody (Monoclonal, 31N04) catalog # M00368-4. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-iNOS/NOS2 Antibody (Monoclonal, 31N04) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00368-4)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
31N04
Immunogen
Synthetic peptide.
Reactive Species
M00368-4 is reactive to NOS2 in Human, Mouse
Calculated molecular weight
131.1 kDa
Background of NOS2
Nitric oxide synthase, inducible is an enzyme that in humans is encoded by the NOS2 gene. Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter; it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. Three different NOS isoforms have been identified which fall into two distinct types, constitutive and inducible. The inducible NOS (iNOS) isoform is expressed in a variety of cell types and tissues in response to inflammatory agents and cytokines. The human iNOS (NOS2) gene is approximately 37 kb in length and consists of 26 exons and 25 introns. NOS2-derived NO is a prerequisite for cytokine signaling and function in innate immunity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00368-4 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Immunocytochemistry/Immunofluorescence, 1:50-200
Validation Images & Assay Conditions
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Effects of MT water extract on DSS-induced UC in zebrafishes. (A) Nontoxic effect of MT water extract (0.2–2 mg/mL) on the development of zebrafishes after 24, 48, and 72 hpf under photomicrograph. (B) Survival rate of zebrafishes. After 72 hpf, zebrafishes were treated with or without 0.5% DSS and co-treated with different concentrations of MT (0.2–2 mg/mL) for 3 days. (C and D) Effects of MT water extract (0.5–1 mg/mL) on the recruitment of neutrophils in DSS-induced IBD in zebrafish larvae. (C) Representative fluorescent images of different groups. Neutrophils recruited in the intestine were highlighted in the white area and zoomed for quantitative analysis (red rectangle). Scale bar: 250 µm. (D) Quantitative analysis of the number of neutrophils recruited in intestine. (E and F) Effects of MT water extract (0.5–1 mg/mL) on DSS-induced ROS production in WT zebrafish larvae. (F) Representative fluorescent images of ROS in different groups stained by CM-H2DCFDA. (F) Quantitative analysis of ROS levels in each group. (G) Effects of MT water extract (0.25–1 mg/mL) on DSS-induced inflammatory gene expressions (iNOS, COX-2, IL-6, and IL-1β) in zebrafish. Data were expressed as mean ± SD from at least three independent trials. One-way ANOVA followed by Tukey's multiple comparison test was performed to compare the differences between groups. *p < 0.05 versus control and #p < 0.05 versus DSS alone group. COX-2, cyclooxygenase-2; DSS, dextran sulfate sodium; ef1α, elongation factor 1α; IL, interleukin; iNOS, inducible nitric oxide synthase; ROS, reactive oxygen species.
Index in Food Frontiers under a CC BY license. DOI: 10.1002/fft2.70164
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Effects of MT water extract on regulating macrophage polarization, maintaining intestinal barrier integrity, and alleviating oxidative stress in the colons of DSS-induced UC mice. (A) IHC staining of iNOS, Arg1, occludin, and ZO-1 expressions in mouse colon tissues. Quantitative analysis of the integrated optical density (IOD) of (B) iNOS, (C) Arg1, (D) occludin, and (E) ZO-1 in each group. The levels of (F) IL-6, (G) IL-10, (H) MDA, and (I) GSH were measured and analyzed. Data were presented as means ± SD for three independent trials. One-way ANOVA followed by Tukey's multiple comparison test was performed to compare the differences between groups. *p < 0.05 versus control and #p < 0.05 versus DSS alone group. Arg1, arginase 1; DSS, dextran sulfate sodium; IL, interleukin; iNOS, inducible nitric oxide synthase; MDA, malondialdehyde; MT, Medulla Tetrapanacis.
Index in Food Frontiers under a CC BY license. DOI: 10.1002/fft2.70164
Specific Publications For Anti-iNOS/NOS2 Antibody (Monoclonal, 31N04) (M00368-4)
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