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Product Info Summary
| SKU: | MA1052 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rabbit, Rat |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10)
SKU/Catalog Number
MA1052
BM0080 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10) catalog # MA1052. Tested in IHC applications. This antibody reacts with Human, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1052)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
K36AC10
Isotype
Mouse IgG1
Immunogen
Human insulin.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1052 is reactive to Ins2 in Human, Rabbit, Rat
Calculated molecular weight
12.3 kDa
Background of Ins2
Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. The insulin gene contains 3 exons and 2 introns; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain. Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Harper et al. (1981) and Harper and Saunders (1981) assigned the insulin gene to 11p15.5 by in situ hybridization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1052 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, rabbit, rat
Positive Control
IHC: Rat Pancreas Tissue, Rat Pancreas Tissue
Validation Images & Assay Conditions
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Anti-Insulin antibody (monoclonal), MA1052, IHC(P)
IHC(P): Rat Pancreas Tissue
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Anti-Insulin antibody (monoclonal), MA1052, IHC(P)
IHC(P): Rat Pancreas Tissue
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HE Staining (×400 magnification) and insulin immunofluorescence staining (×200 magnification) in a rat pancreatic islet . Islet cells in the NC (A1 and B1) and CUGFR (A2 and B2) groups 4 weeks after catch-up growth with HE staining (A1 and A2) and insulin fluorescence staining (B1 and B2). Green fluorescence shows cells positive for insulin.
Index in PubMed under a CC BY license. PMID: 20504302
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Effect of catch-up growth on beta cell relative area and mass . After insulin staining of rat pancreatic islet, the area positively stained for insulin was quantified by Image Pro Plus software. Values are means ± SD (n = 6). *P < 0.05 versus NC group. **P < 0.01 versus NC group.
Index in PubMed under a CC BY license. PMID: 20504302
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Effect of catch-up growth on islet function and GLP-1 concentration . Plasma glucose (A), insulin (B), GLP-1(C) levels and AUC Insulin , AUC Glucose , AUC GLP-1 during a 1-hour OGTT, which was conducted 0 (A1, B1 and C1), 2 (A2, B2 and C2) and 4 (A3, B3 and C3) weeks after catch-up growth in the NC (red) and CUGFR (green) groups, were measured. Data are expressed as means ± SD of six rats in each group. *P ≤ 0.05 versus NC group; **P ≤ 0.01 versus NC group.
Index in PubMed under a CC BY license. PMID: 20504302
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Time course of the glucose and insulin following oral and IV glucose in the normal and catch-up growth rats . Plasma glucose (A1, A2, A3) and insulin (B1, B2, B3) concentrations in rats of NC group (red) or CUGFR group (green) during oral glucose tolerance test (solid line) and intravenous glucose infusion test (broken lines) designed to match the oral glucose curve in six rats, were detected after catch up growth for 0 (A1, B1), 2(A2, B2) and 4 (A3, B3) weeks. Data are expressed as means ± SD.
Index in PubMed under a CC BY license. PMID: 20504302
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Effect of catch-up growth on the incretin effect . Data are expressed as means ± SD. The incretin effect was calculated by comparing the insulin response to oral and matched IV glucose load. * P ≤ 0.05 versus NC group.
Index in PubMed under a CC BY license. PMID: 20504302
Specific Publications For Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10) (MA1052)
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Customer Q&As
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7 Customer Q&As for Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10)
Question
Do you have a BSA free version of anti-Insulin antibody (Monoclonal, K36AC10) MA1052 available?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Insulin antibody (Monoclonal, K36AC10) MA1052 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-19
Question
We are currently using anti-Insulin antibody (Monoclonal, K36AC10) MA1052 for rabbit tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rabbit, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The anti-Insulin antibody (Monoclonal, K36AC10) (MA1052) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-27
Question
See attached the WB image, lot number and protocol we used for pancreas using anti-Insulin antibody (Monoclonal, K36AC10) MA1052. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-19
Question
Will anti-Insulin antibody (Monoclonal, K36AC10) MA1052 work on goat IHC with blood?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
Our lab technicians have not tested anti-Insulin antibody (Monoclonal, K36AC10) MA1052 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Insulin antibody (Monoclonal, K36AC10) MA1052 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat blood in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-31
Question
We need to test anti-Insulin antibody (Monoclonal, K36AC10) MA1052 on rat pancreas for research purposes, then I may be interested in using anti-Insulin antibody (Monoclonal, K36AC10) MA1052 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-09-04
Answer
The products we sell, including anti-Insulin antibody (Monoclonal, K36AC10) MA1052, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-04
Question
I see that the anti-Insulin antibody (Monoclonal, K36AC10) MA1052 works with IHC, what is the protocol used to produce the result images on the product page?
V. Gonzalez
Verified customer
Asked: 2016-01-07
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-01-07
Question
Can you help my question with product MA1052, anti-Insulin antibody (Monoclonal, K36AC10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Evans
Verified customer
Asked: 2013-03-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1052 anti-Insulin antibody (Monoclonal, K36AC10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-03-01
