Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10)

Ins2 antibody

Boster Bio Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10) catalog # MA1052. Tested in IHC applications. This antibody reacts with Human, Rabbit, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: MA1052
Size: 100 μg/vial
Reactive Species: Human, Rabbit, Rat
Host: Mouse
Application: IHC

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Product Name

Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10)

View all Ins2 Antibodies

SKU/Catalog Number



100 μg/vial




Boster Bio Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10) catalog # MA1052. Tested in IHC applications. This antibody reacts with Human, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1052)




Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.



Clone Number



Mouse IgG1


Human insulin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1052 is reactive to Ins2 in Human, Rabbit, Rat


MA1052 is guaranteed for IHC Boster Guarantee

Background of Ins2

Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulfide bonds. The insulin gene contains 3 exons and 2 introns; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain. Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Harper et al. (1981) and Harper and Saunders (1981) assigned the insulin gene to 11p15.5 by in situ hybridization.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, rabbit, rat, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For Ins2 (Source:, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name





insulin family

Alternative Names

Insulin-2 Ins2|CP-II|insulin 2|insulin-2|C-peptide

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Ins2, check out the Ins2 Infographic

Ins2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Ins2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

MA1052 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Gao T,Jiao Y,Liu Y,Li T,Wang Z,Wang D.Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes.J Diabetes Res.2019 Oct 7;2019:3872182.doi:10.1155/2019/3872182.PMID:31687407;PMCID:PMC6800947.
Species: Rat
MA1052 usage in article: APP:IHC, SAMPLE:PANCREASE, DILUTION:1:200

Dong X,Zhao SX,Yin XL,Wang HY,Wei ZG,Zhang YQ.Silk sericin has significantly hypoglycaemic effect in type 2 diabetic mice via anti-oxidation and anti-inflammation.Int J Biol Macromol.2020 May 1;150:1061-1071.doi:10.1016/j.ijbiomac.2019.10.111.Epub 2019 Nov 16.PMID:31743716.
Species: Mouse

Huo K,Li X,Hu W,Song X,Zhang D,Zhang X,Chen X,Yuan J,Zuo J,Wang X.RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis.Front Endocrinol (Lausanne). 2020 Apr 9;11:194.doi:10.3389/fendo.2020.00194.PMID
Species: Rat

Zhou D, Chen L, Mou X. Acarbose ameliorates spontaneous type‑2 diabetes in db/db mice by inhibiting PDX‑1 methylation. Mol Med Rep. 2021 Jan;23(1):72. doi: 10.3892/mmr.2020.11710. Epub 2020 Nov 25. PMID: 33236139; PMCID: PMC7716388.
Species: Mouse

miR-487b mitigates chronic heart failure through inhibition of the IL-33/ST2 signaling pathway

Liu D, Zhou Y, Peng Y, Su P, Li Z, Xu Q, Tu Y, Tian X, Yang H, Wu Z, Mei W, Gao F. Front Mol Neurosci. 2018 Mar 6;11:72. doi: 10.3389/fnmol.2018.00072. eCollection 2018. Endoplasmic Reticulum Stress in Spinal Cord Contributes to the Development of...

Xu F, Dong Y, Huang X, Li M, Qin L, Ren Y, Guo F, Chen A, Huang S. Mol Med Rep. 2014 Oct;10(4):1935-41. Doi: 10.3892/Mmr.2014.2430. Epub 2014 Jul 30. Decreased Osteoclastogenesis, Osteoblastogenesis And Low Bone Mass In A Mouse Model Of Type 2 Dia...

Su X, Meng X, Sun C, Liu L, Su B. J Diabetes. 2011 Dec;3(4):309-16. Doi: 10.1111/J.1753-0407.2011.00153.X. Intramuscular Injection Of Soluble Receptor For Advanced Glycation Endproducts Expression Vector Prevents The Development Of Streptozotocin-...

Shen L, Liu X, Hou W, Yang G, Wu Y, Zhang R, Li X, Che H, Lu Z, Zhang Y, Liu X, Yao L. Cell Mol Life Sci. 2010 Apr;67(8):1371-81. Doi: 10.1007/S00018-010-0258-1. Epub 2010 Feb 3. Ndrg2 Is Highly Expressed In Pancreatic Beta Cells And Involved In P...

Xu Y, Ke H, Liang L, Wang X, Pei J, Du L. Horm Res Paediatr. 2011;76(1):35-43. Doi: 10.1159/000324566. Epub 2011 Mar 10. Peg-Rhgh Therapy For Small For Gestational Age Rats And Effects On Their Metabolic Parameters.

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7 Customer Q&As for Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10)


Do you have a BSA free version of anti-Insulin antibody (Monoclonal, K36AC10) MA1052 available?

Verified Customer

Verified customer

Asked: 2020-02-19


I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Insulin antibody (Monoclonal, K36AC10) MA1052 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-19


We are currently using anti-Insulin antibody (Monoclonal, K36AC10) MA1052 for rabbit tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rabbit, rat. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-27


The anti-Insulin antibody (Monoclonal, K36AC10) (MA1052) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-27


See attached the WB image, lot number and protocol we used for pancreas using anti-Insulin antibody (Monoclonal, K36AC10) MA1052. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-19


Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-19


Will anti-Insulin antibody (Monoclonal, K36AC10) MA1052 work on goat IHC with blood?

Verified Customer

Verified customer

Asked: 2018-01-31


Our lab technicians have not tested anti-Insulin antibody (Monoclonal, K36AC10) MA1052 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Insulin antibody (Monoclonal, K36AC10) MA1052 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat blood in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-31


We need to test anti-Insulin antibody (Monoclonal, K36AC10) MA1052 on rat pancreas for research purposes, then I may be interested in using anti-Insulin antibody (Monoclonal, K36AC10) MA1052 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-09-04


The products we sell, including anti-Insulin antibody (Monoclonal, K36AC10) MA1052, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-09-04


I see that the anti-Insulin antibody (Monoclonal, K36AC10) MA1052 works with IHC, what is the protocol used to produce the result images on the product page?

V. Gonzalez

Verified customer

Asked: 2016-01-07


You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-01-07


Can you help my question with product MA1052, anti-Insulin antibody (Monoclonal, K36AC10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Evans

Verified customer

Asked: 2013-03-01


We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1052 anti-Insulin antibody (Monoclonal, K36AC10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-03-01



Total: $315


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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)

$50 fee for conjugation. Antibody size is reduced to 50ug.



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