Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10)

Boster Bio Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10) catalog # MA1052. Tested in IHC applications. This antibody reacts with Human, Rabbit, Rat. Cited in 25 publication(s).

Product Info Summary

SKU: MA1052
Size: 100μg/vial
Reactive Species: Human, Rabbit, Rat
Host: Mouse
Application: IHC

Product Name

Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10)

See all Ins2 products

SKU/Catalog Number

MA1052

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10) catalog # MA1052. Tested in IHC applications. This antibody reacts with Human, Rabbit, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1052)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

K36AC10

Isotype

Mouse IgG1

Immunogen

Human insulin.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1052 is reactive to Ins2 in Human, Rabbit, Rat

Applications

MA1052 is guaranteed for IHC Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, rabbit, rat, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For Ins2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P01323

Gene ID

24506

Gene Name

Ins2

Full Name

Insulin-2

Weight

12.339kDa

Superfamily

insulin family

Alternative Names

Insulin-2 Ins2|CP-II|insulin 2|insulin-2|C-peptide

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ins2, check out the Ins2 Infographic

Ins2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ins2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1052 has been cited in 25 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Gao T,Jiao Y,Liu Y,Li T,Wang Z,Wang D.Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes.J Diabetes Res.2019 Oct 7;2019:3872182.doi:10.1155/2019/3872182.PMID:31687407;PMCID:PMC6800947.
Species: Rat
MA1052 usage in article: APP:IHC, SAMPLE:PANCREASE, DILUTION:1:200

Dong X,Zhao SX,Yin XL,Wang HY,Wei ZG,Zhang YQ.Silk sericin has significantly hypoglycaemic effect in type 2 diabetic mice via anti-oxidation and anti-inflammation.Int J Biol Macromol.2020 May 1;150:1061-1071.doi:10.1016/j.ijbiomac.2019.10.111.Epub 2019 Nov 16.PMID:31743716.
Species: Mouse
MA1052 usage in article: APP:IHC, SAMPLE:LIVER AND PANCREASE TISSUES, DILUTION:NA

Huo K,Li X,Hu W,Song X,Zhang D,Zhang X,Chen X,Yuan J,Zuo J,Wang X.RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis.Front Endocrinol (Lausanne). 2020 Apr 9;11:194.doi:10.3389/fendo.2020.00194.PMID
Species: Rat
MA1052 usage in article: APP:IHC, SAMPLE:PANCREATIC TISSUE, DILUTION:1:100

Zhou D, Chen L, Mou X. Acarbose ameliorates spontaneous type‑2 diabetes in db/db mice by inhibiting PDX‑1 methylation. Mol Med Rep. 2021 Jan;23(1):72. doi: 10.3892/mmr.2020.11710. Epub 2020 Nov 25. PMID: 33236139; PMCID: PMC7716388.
Species: Mouse
MA1052 usage in article: APP:IHC, SAMPLE:PANCREATIC TISSUE, DILUTION:1:50

miR-487b mitigates chronic heart failure through inhibition of the IL-33/ST2 signaling pathway

Liu D, Zhou Y, Peng Y, Su P, Li Z, Xu Q, Tu Y, Tian X, Yang H, Wu Z, Mei W, Gao F. Front Mol Neurosci. 2018 Mar 6;11:72. doi: 10.3389/fnmol.2018.00072. eCollection 2018. Endoplasmic Reticulum Stress in Spinal Cord Contributes to the Development of...

Xu F, Dong Y, Huang X, Li M, Qin L, Ren Y, Guo F, Chen A, Huang S. Mol Med Rep. 2014 Oct;10(4):1935-41. Doi: 10.3892/Mmr.2014.2430. Epub 2014 Jul 30. Decreased Osteoclastogenesis, Osteoblastogenesis And Low Bone Mass In A Mouse Model Of Type 2 Dia...

Su X, Meng X, Sun C, Liu L, Su B. J Diabetes. 2011 Dec;3(4):309-16. Doi: 10.1111/J.1753-0407.2011.00153.X. Intramuscular Injection Of Soluble Receptor For Advanced Glycation Endproducts Expression Vector Prevents The Development Of Streptozotocin-...

Shen L, Liu X, Hou W, Yang G, Wu Y, Zhang R, Li X, Che H, Lu Z, Zhang Y, Liu X, Yao L. Cell Mol Life Sci. 2010 Apr;67(8):1371-81. Doi: 10.1007/S00018-010-0258-1. Epub 2010 Feb 3. Ndrg2 Is Highly Expressed In Pancreatic Beta Cells And Involved In P...

Xu Y, Ke H, Liang L, Wang X, Pei J, Du L. Horm Res Paediatr. 2011;76(1):35-43. Doi: 10.1159/000324566. Epub 2011 Mar 10. Peg-Rhgh Therapy For Small For Gestational Age Rats And Effects On Their Metabolic Parameters.

Adise S, Saliu A, Maldonado N, Khatri V, Cardoso L, Rodr??guez-Contreras A. J Neurosci. 2014 Mar 26;34(13):4528-33. Doi: 10.1523/Jneurosci.4188-13.2014. Effect Of Maternal Care On Hearing Onset Induced By Developmental Changes In The Auditory Peri...

Lu J, Gu Yp, Xu X, Liu Ml, Xie P, Song Hp. World J Gastroenterol. 2005 Jun 14;11(22):3426-30. Adult Islets Cultured In Collagen Gel Transdifferentiate Into Duct-Like Cells.

Liang H, Yin B, Zhang H, Zhang S, Zeng Q, Wang J, Jiang X, Yuan L, Wang Cy, Li Z. Endocrinology. 2008 Jun;149(6):2943-51. Doi: 10.1210/En.2007-0978. Epub 2008 Mar 13. Blockade Of Tumor Necrosis Factor (Tnf) Receptor Type 1-Mediated Tnf-Alpha Signa...

Zhou J, Zhou S, Zeng S. Fundam Clin Pharmacol. 2013 Jun;27(3):279-87. Doi: 10.1111/J.1472-8206.2011.01022.X. Epub 2011 Dec 16. Experimental Diabetes Treated With Trigonelline: Effect On ?? Cell And Pancreatic Oxidative Parameters.

Wang X, Ni S, Xu Y, Liang L, Du L, Gu W. Endocr Res. 2012;37(3):97-109. Doi: 10.3109/07435800.2011.635621. Epub 2012 May 23. Effects Of Long-Term High-Fat/High-Energy And High-Protein Diets On Insulin And Ghrelin Expression In Developing Rats.

Hu N, Yuan L, Li Hj, Huang C, Mao Qm, Zhang Yy, Lin M, Sun Yq, Zhong Xy, Tang P, Lu X. Evid Based Complement Alternat Med. 2013;2013:180721. Doi: 10.1155/2013/180721. Epub 2013 May 29. Anti-Diabetic Activities Of Jiaotaiwan In Db/Db Mice By Augmen...

Gao Lh, Liu Q, Liu Sn, Chen Zy, Li Cn, Lei L, Sun Sj, Li Ly, Liu Jl, Shen Zf. J Ethnopharmacol. 2014;151(1):675-85. Doi: 10.1016/J.Jep.2013.11.024. Epub 2013 Nov 25. A Refined-Jinqi-Jiangtang Tablet Ameliorates Prediabetes By Reducing Insulin Resi...

Ding H, Zhu T, Yin X, Liu J, Zhang L, Bernier M, Zhao R. Acta Biochim Biophys Sin (Shanghai). 2014 Jul;46(7):582-9. Doi: 10.1093/Abbs/Gmu034. Epub 2014 May 14. Pyrrolidine Dithiocarbamate Protects Pancreatic ??-Cells From Oxidative Damage Through ...

Chen Ll, Yang Wh, Zheng J, Hu X, Kong W, Zhang Hh. Nutr Metab (Lond). 2010 May 26;7:45. Doi: 10.1186/1743-7075-7-45. Effect Of Catch-Up Growth After Food Restriction On The Entero-Insular Axis In Rats.

Jiang H, Deng Xf, Duan Cm, Chen C, Xiang Jl, Lu Yl, Ma Qf. Lymphology. 2011 Mar;44(1):21-8. Somatostatin Receptors Sstr2 And Sstr5 Are Expressed In The Human Thoracic Duct.

Zhang Y, Ye C, Wang G, Gao Y, Tan K, Zhuo Z, Liu Z, Xia H, Yang D, Li P. Biomed Res Int. 2013;2013:526367. Doi: 10.1155/2013/526367. Epub 2013 May 27. Kidney-Targeted Transplantation Of Mesenchymal Stem Cells By Ultrasound-Targeted Microbubble Des...

Zhang W, Jin H, Hu Y, Yu Y, Li X, Ding Z, Kang Y, Wang B. Hum Gene Ther. 2010 Feb;21(2):171-8. Doi: 10.1089/Hum.2009.095. Protective Response Against Type 1 Diabetes In Nonobese Diabetic Mice After Coimmunization With Insulin And Dna Encoding Proi...

Liu Jj, Raynal S, Bailb?? D, Gausseres B, Carbonne C, Autier V, Movassat J, Kergoat M, Portha B. Biochim Biophys Acta. 2015 May;1852(5):980-91. Doi: 10.1016/J.Bbadis.2015.02.001. Epub 2015 Feb 9. Expression Of The Kynurenine Pathway Enzymes In The...

Liu L, Gu H, Zhao Y, An L, Yang J. Exp Ther Med. 2014 Dec;8(6):1813-1818. Epub 2014 Sep 30. Glypican 4 May Be Involved In The Adipose Tissue Redistribution In High-Fat Feeding C57Bl/6J Mice With Peroxisome Proliferators-Activated Receptor ?? Agoni...

Han X, Qiu L, Zhang Y, Kong Q, Wang H, Wang H, Li H, Duan C, Wang Y, Song Y, Wang C. Exp Biol Med (Maywood). 2009 May;234(5):595-603. Doi: 10.3181/0812-Rm-359. Epub 2009 Feb 25. Transplantation Of Sertoli-Islet Cell Aggregates Formed By Microgravi...

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7 Customer Q&As for Anti-Insulin Ins2 Antibody (Monoclonal, K36AC10)

Question

Do you have a BSA free version of anti-Insulin antibody (Monoclonal, K36AC10) MA1052 available?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Insulin antibody (Monoclonal, K36AC10) MA1052 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-19

Question

We are currently using anti-Insulin antibody (Monoclonal, K36AC10) MA1052 for rabbit tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rabbit, rat. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

The anti-Insulin antibody (Monoclonal, K36AC10) (MA1052) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-27

Question

See attached the WB image, lot number and protocol we used for pancreas using anti-Insulin antibody (Monoclonal, K36AC10) MA1052. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-19

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-19

Question

Will anti-Insulin antibody (Monoclonal, K36AC10) MA1052 work on goat IHC with blood?

Verified Customer

Verified customer

Asked: 2018-01-31

Answer

Our lab technicians have not tested anti-Insulin antibody (Monoclonal, K36AC10) MA1052 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Insulin antibody (Monoclonal, K36AC10) MA1052 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat blood in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-31

Question

We need to test anti-Insulin antibody (Monoclonal, K36AC10) MA1052 on rat pancreas for research purposes, then I may be interested in using anti-Insulin antibody (Monoclonal, K36AC10) MA1052 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-09-04

Answer

The products we sell, including anti-Insulin antibody (Monoclonal, K36AC10) MA1052, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-09-04

Question

I see that the anti-Insulin antibody (Monoclonal, K36AC10) MA1052 works with IHC, what is the protocol used to produce the result images on the product page?

V. Gonzalez

Verified customer

Asked: 2016-01-07

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-01-07

Question

Can you help my question with product MA1052, anti-Insulin antibody (Monoclonal, K36AC10). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Evans

Verified customer

Asked: 2013-03-01

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1052 anti-Insulin antibody (Monoclonal, K36AC10), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-03-01

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