Human Cystatin D/CST5 ELISA Kit PicoKine™

Human Cystatin D/CST5 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CST5 in cell culture supernatants, serum and plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1964
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA) , saliva and urine.

Product Name

Human Cystatin D/CST5 ELISA Kit PicoKine™

See all Cystatin D/Cst5 products

SKU/Catalog Number

EK1964

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Cystatin D/CST5 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CST5 in cell culture supernatants, serum and plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Cystatin D/CST5 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1964)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G21-V142

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1964 is reactive to CST5 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA) , saliva and urine.

Application Guarantee

EK1964 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1964-CAP Anti-Human CST5 Pre-coated 96-well strip microplate 1
EK1964-ST Human CST5 Standard 2 vials, 10 ng/tube
EK1964-DA Human CST5 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0130.0630.1130.2080.3610.7131.2112.038

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Cystatin D/Cst5, Dilution ratio of 1:1, concentration in serum is around 80pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Cystatin D/CST5 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3021545631287532
Standard deviation1.2611.40 25.991.9216.6533.52
CV (%)4.2%5.3%5.7%6.2%5.8%6.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cystatin D/Cst5 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 130312727281.786.3%
Sample 221521820223121610.304.7%
Sample 345646143551846730.746.5%
*number of samples for each test n=16.

Gene/Protein Information For CST5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P28325

Gene ID

1473

Gene Name

CST5

Full Name

Cystatin-D

Weight

16.08kDa

Superfamily

cystatin family

Alternative Names

CST5; CSTD; cystatin 5; Cystatin D; Cystatin-5; cystatin-D; cysteine-proteinase inhibitor; MGC71922 CST5 cystatin D cystatin-D|cystatin 5|cysteine-proteinase inhibitor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CST5, check out the CST5 Infographic

CST5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CST5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1964

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7 Customer Q&As for Human Cystatin D/CST5 ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated Cystatin D antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cystatin D antigen?

J. Gandhi

Verified customer

Asked: 2020-09-22

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-09-22

Question

Q: Can Cystatin D ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-06-11

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-06-11

Question

A question regarding our previous contact about EK1964: do you have any recommended dilutions for serum and urine samples? I couldn

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

Recommended dilution for serum and urine samples is 1:1.

Boster Scientific Support

Answered: 2020-02-21

Question

Q: can you recommend the dilution ratio of serum samples for detection of Cystatin D in Human plasma ? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

B. Thompson

Verified customer

Asked: 2019-11-11

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Cystatin D ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-11-11

Question

What is the recommended dilution ratios for serum and urine samples using EK1964?

Verified customer

Asked: 2019-08-01

Answer

The recommended dilution for serum and urine samples using the Human Cystatin D/CST5 ELISA Kit PicoKine™ (EK1964) is 1:1.

Boster Scientific Support

Answered: 2019-08-01

Question

Q: What is the optimal O.D. value for Cystatin D ELISA kit? I used your Cystatin D ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Cystatin D even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Cystatin D ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Cystatin D that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-05-14

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Cystatin D using the EK1964 Human Cystatin D Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-02-02

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2019-02-02

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