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IRF2BPL antibody (A10706) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HEL cells using anti-IRF2BPL antibody (A10706).
Overlay histogram showing HEL cells stained with A10706 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRF2BPL Antibody (A10706, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEL cells stained with A10706 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRF2BPL Antibody (A10706, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-IRF2BPL Antibody Picoband®
IRF2BPL antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
IRF2BPL antibody was positively detected by WB in:
human PC-3 whole cell, human SH-SY5Y whole cell, human MCF-7whole cell, human RT4 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
IRF2BPL antibody was positively detected by IHC in:
follicles of human thyroid cancer tissue, follicles of human thyroid cancer tissue, follicles of human glioma tissue, follicles of human glioma tissue, follicles of human thyroid cancer tissue, follicles of human thyroid cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
IRF2BPL antibody was positively detected by ICC/IF in:
TPC1 cell
IRF2BPL antibody was positively detected by FC in:
HEL cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Rat
Predicted
Predicted
Bovine
Predicted
Predicted
Mouse
Predicted
Predicted
Sheep
Predicted
Predicted
Product Information
Product Name
Anti-IRF2BPL Antibody Picoband®
View all IRF2BPL Antibodies
View all IRF2BPL Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human IRF2BPL recombinant protein (Position: R472-R721). Human IRF2BPL shares 98.4% and 99.2% amino acid (aa) sequence identity with mouse and rat IRF2BPL, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
95 kDa
Calculated Molecular Weight
82.7 kDa
Background of IRF2BPL
Interferon regulatory factor 2 binding protein like is a protein that in humans is encoded by the IRF2BPL gene. This gene encodes a transcription factor that may play a role in regulating female reproductive function.
Cite This Product
Anti-IRF2BPL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10706)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rat
99%
High Homology
Cow
99%
High Homology
Mouse
98%
High Homology
Sheep
98%
High Homology
Rabbit
74%
Moderate Homology
Zebrafish
69%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for IRF2BPL antibody
Immunohistochemistry (IHC) protocol for IRF2BPL antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for IRF2BPL antibody
Flow Cytometry (FC) protocol for IRF2BPL antibody
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Product Promise
We promise Anti-IRF2BPL Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A10706
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026