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Product Info Summary
| SKU: | A02772 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-IZUMO1 Antibody
SKU/Catalog Number
A02772
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IZUMO1 Antibody (Catalog # A02772). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human.
Storage & Handling
IZUMO1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-IZUMO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02772)
Host
Rabbit
Contents
IZUMO1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IZUMO1 antibody was raised against an 18 amino acid peptide near the center of human IZUMO1. The immunogen is located within amino acids 170 - 220 of IZUMO1.
Cross-reactivity
IZUMO1 antibody is human specific. At least four isoforms of IZUMO1 are known to exist; this antibody will detect the two longest isoforms.
Reactive Species
A02772 is reactive to IZUMO1 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
38.9 kDa
Background of IZUMO1
IZUMO1, a sperm-specific protein, is essential for sperm-egg plasma membrane binding and fusion. It is involved in the fertilization process and in the fusion of the sperm with the egg (1,2). IZUMO1 is expressed as at least four isoforms produced by alternative splicing and contains one Ig-like (immunoglobulin-like) C2-type domain (3). Lack of IZUMO1 in males, due to homozygous mutation in the gene that encodes IZUMO1, results in sterility due to an inability to penetrate the plasma membrane of the egg (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02772 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IZUMO1 antibody can be used for detection of IZUMO1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IZUMO1 in human testis tissue lysate with IZUMO1 antibody at 1 μg/ml.
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Immunohistochemistry of IZUMO1 in human testis tissue with IZUMO1 antibody at 2.5 μg/mL.
Specific Publications For Anti-IZUMO1 Antibody (A02772)
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